Omi T, Kajii E, Iwamoto S, Tsuchida S, Ikemoto S
Department of Legal Medicine and Human Genetics, Jichi Medical School, Tochigi, Japan.
Electrophoresis. 1994 Aug-Sep;15(8-9):1091-4. doi: 10.1002/elps.11501501163.
Two truncated mRNAs, in contrast to the full length of mRNA associated with the glycophorin A gene (GpA-TI, GpA-TII), were isolated from erythroid cells cultured by the selective two-phase liquid culture system for erythroid progenitors in peripheral blood from a normal individual. The GpA-TI mRNAs displayed a direct transition from exon I to exon III, so that the deletion of exon II resulted in the deletion of 33 amino acids encoded by this exon. Furthermore, the GpA-TII showed two direct transitions from exon I to exon III and from exon III to the exon V of the GpA gene. This mRNA lacked both exons II and VI, resulting in the deletion of 46 amino acids. Is is concluded that these truncated mRNAs are transcribed from the same gene as the GpA gene and correspond to splicing isoforms lacking different exons.
与正常个体外周血红细胞祖细胞选择性两相液体培养系统培养的红细胞中分离出的与血型糖蛋白A基因（GpA - TI、GpA - TII）相关的全长mRNA相比，有两种截短的mRNA。GpA - TI mRNA显示从外显子I直接过渡到外显子III，因此外显子II的缺失导致该外显子编码的33个氨基酸的缺失。此外，GpA - TII显示从外显子I到外显子III以及从外显子III到GpA基因外显子V的两个直接过渡。这种mRNA既缺乏外显子II也缺乏外显子VI，导致46个氨基酸的缺失。得出的结论是，这些截短的mRNA与GpA基因转录自同一基因，并且对应于缺乏不同外显子的剪接异构体。
