用于小鼠脑中去甲肾上腺素和3-甲氧基-4-羟基苯乙二醇的带库仑检测的快速高效液相色谱法。

Rapid HPLC assay with coulometric detection for norepinephrine and 3-methoxy-4-hydroxyphenylglycol in the mouse brain.

作者信息

Murai S, Saito H, Masuda Y, Ohkubo N, Itoh T

机构信息

Department of Pharmacology, School of Dentistry, Iwate Medical University, Morioka, Japan.

出版信息

J Pharmacol Toxicol Methods. 1994 Oct;32(2):99-103. doi: 10.1016/1056-8719(94)90060-4.

DOI:10.1016/1056-8719(94)90060-4

PMID:7532470

Abstract

For the rapid assay of norepinephrine (NE) and its major metabolite, 3-methoxy-4-hydroxyphenylglycol (MHPG) in the mouse brain, we developed a simple method using isocratic HPLC with coulometric detection. This method permits NE and MHPG assay within 5 min in one chromatographic run. Within-run coefficients of variation for NE and MHPG in the working standard solution were 0.8% and 0.6% (n = 50), respectively. The detector responses were linear from 0.025 to 100 pmol for NE and from 0.05 to 100 pmol for MHPG in the working standard solution. Using this method, the NE and MHPG concentrations were measured in discrete brain areas of the mouse prior treatment with or without alpha-methyl-p-tyrosine or N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP4).

摘要

为了快速测定小鼠脑中的去甲肾上腺素（NE）及其主要代谢产物3-甲氧基-4-羟基苯乙二醇（MHPG），我们开发了一种使用等度高效液相色谱法（HPLC）结合库仑检测的简单方法。该方法可在一次色谱运行中5分钟内完成NE和MHPG的测定。工作标准溶液中NE和MHPG的批内变异系数分别为0.8%和0.6%（n = 50）。在工作标准溶液中，NE的检测响应在0.025至100 pmol范围内呈线性，MHPG的检测响应在0.05至100 pmol范围内呈线性。使用该方法，在给予或未给予α-甲基-p-酪氨酸或N-（2-氯乙基）-N-乙基-2-溴苄胺（DSP4）预处理的小鼠离散脑区中测量了NE和MHPG的浓度。