Dzoljic M R, De Vries R
Department of Pharmacology, Faculty of Medicine and Health Sciences, Erasmus University Rotterdam, The Netherlands.
Neuropharmacology. 1994 Nov;33(11):1505-9. doi: 10.1016/0028-3908(94)90055-8.
The effect of NG-monomethyl-L-arginine (L-NMMA), an inhibitor of nitric oxide (NO) synthase and L-arginine, a precursor of NO, was examined on the sleep-waking pattern in rats. L-NMMA (3.75-15 mg/kg, i.p.) reduced wakefulness with a corresponding increase of slow wave sleep and rapid eye movement sleep. The effect of L-NMMA on vigilance was limited to the first hour following drug administration. The effect of L-NMMA was abolished by intracerebroventricular administration of L-arginine (600 micrograms). This indicates that the inhibitory effect of L-NMMA on wakefulness is mediated by decreased NO synthesis and that central NO exerts an excitatory role in vigilance. It further implicates that factors facilitating a release and/or synthesis of NO might lead to increased wakefulness and sleep disturbances.
研究了一氧化氮（NO）合酶抑制剂NG-单甲基-L-精氨酸（L-NMMA）和NO前体L-精氨酸对大鼠睡眠-觉醒模式的影响。L-NMMA（3.75-15毫克/千克，腹腔注射）减少了觉醒，同时慢波睡眠和快速眼动睡眠相应增加。L-NMMA对警觉性的影响仅限于给药后的第一小时。脑室内注射L-精氨酸（600微克）可消除L-NMMA的作用。这表明L-NMMA对觉醒的抑制作用是通过减少NO合成介导的，并且中枢NO在警觉性中发挥兴奋作用。这进一步表明，促进NO释放和/或合成的因素可能导致觉醒增加和睡眠障碍。
