Macedo A, Orfao A, Martínez A, Vidriales M B, Valverde B, López-Berges M C, San Miguel J F
Servicio de Hematología, Hospital Clínico Universitario, Salamanca, Spain.
Br J Haematol. 1995 Feb;89(2):338-41. doi: 10.1111/j.1365-2141.1995.tb03309.x.
In the present study, seven normal human bone marrow samples from healthy volunteers have been analysed in order to investigate the immunophenotypic characteristics of the normal CD117+ cells and their utility for the detection of minimal residual disease in 71 acute myeloid leukaemia patients. Our results show that most of normal BM CD117+ cells coexpress the HLADR and the myeloid associated CD33 antigen. In addition, almost half of CD117+ cells are CD34+, these cells displaying a different FSC/SSC distribution when compared to the CD117+/CD34- cells. No CD117+/CD15+ and CD117+/CD10+ cells were detected and very few CD117+ cells (< 1 x 10(-3) expressing the HLADR-/CD34-, CD33+/HLADR- and CD34+/HLADR- phenotypes were found to be present in normal BM. In contrast, from the 71 AML patients analysed, 34 had CD117+/CD15+ blast cells and eight had the CD117+ phenotypes detected at low frequencies (< 1 x 10(-3)) in normal BM. In summary, the present study shows that the use of the CD117 antigen in different monoclonal antibodies combinations may be of great help for the detection of minimal residual disease in a high proportion of AML cases, especially in those patients displaying the CD117+/CD15+ phenotype, because cells coexpressing both antigens in normal BM, if present, are at very low frequencies.
在本研究中，对来自健康志愿者的7份正常人骨髓样本进行了分析，以研究正常CD117+细胞的免疫表型特征及其在检测71例急性髓系白血病患者微小残留病中的作用。我们的结果显示，大多数正常骨髓CD117+细胞共表达HLA-DR和髓系相关的CD33抗原。此外，几乎一半的CD117+细胞为CD34+，与CD117+/CD34-细胞相比，这些细胞呈现出不同的FSC/SSC分布。未检测到CD117+/CD15+和CD117+/CD10+细胞，在正常骨髓中发现极少数表达HLA-DR-/CD34-、CD33+/HLA-DR-和CD34+/HLA-DR-表型的CD117+细胞（<1×10⁻³）。相反，在分析的71例AML患者中，34例有CD117+/CD15+原始细胞，8例在正常骨髓中检测到低频（<1×10⁻³）的CD117+表型。总之，本研究表明，在不同单克隆抗体组合中使用CD117抗原可能对检测高比例的AML病例中的微小残留病有很大帮助，特别是在那些显示CD117+/CD15+表型的患者中，因为正常骨髓中同时表达这两种抗原的细胞（如果存在）频率非常低。
