Schmitz B, Schachner M, Ito Y, Nakano T, Ogawa T
Department of Neurobiology, Swiss Federal Institute of Technology, Zürich.
Glycoconj J. 1994 Aug;11(4):345-52. doi: 10.1007/BF00731208.
The L2/HNK-1 carbohydrate epitope has been shown to carry an unusual 3'-sulfoglucuronic acid linked O-glycosidically through a neolactosyl-type backbone to a ceramide residue. Using monoclonal antibodies, the same or a closely related epitope has also been detected N-glycosidically linked to glycoproteins, amongst them several neural cell adhesion molecules. We used synthetic glycolipids carrying sulfated or non-sulfated glucuronic acid attached to ceramide through glycans of different length to show that not only the sulfated glucuronic acid but also the neolactosyl-type backbone is essential for the recognition of the L2/HNK-1 carbohydrate by a monoclonal antibody, its binding to laminin and its role in neural cell migration and outgrowth of processes from neurons and astrocytes.
已证实L2/HNK-1碳水化合物表位带有一个不寻常的3'-硫酸化葡萄糖醛酸，它通过新乳糖基型主链以O-糖苷键连接到神经酰胺残基上。使用单克隆抗体，还检测到相同或密切相关的表位以N-糖苷键连接到糖蛋白上，其中包括几种神经细胞粘附分子。我们使用了通过不同长度聚糖连接到神经酰胺上的带有硫酸化或非硫酸化葡萄糖醛酸的合成糖脂，以表明不仅硫酸化葡萄糖醛酸，而且新乳糖基型主链对于单克隆抗体识别L2/HNK-1碳水化合物、其与层粘连蛋白的结合以及其在神经细胞迁移以及神经元和星形胶质细胞突起生长中的作用都是必不可少的。
