Ellis N A, Tippett P, Petty A, Reid M, Weller P A, Ye T Z, German J, Goodfellow P N, Thomas S, Banting G
New York Blood Center, New York 10021.
Nat Genet. 1994 Nov;8(3):285-90. doi: 10.1038/ng1194-285.
We have identified the Xga antigen, encoded by the XG blood group gene, by employing rabbit polyclonal and mouse monoclonal antibodies raised against a peptide derived from the N-terminal domain of a candidate gene, referred to earlier as PBDX. In indirect haemagglutination assays, these anti-peptide antibodies react with Xg(a+) but not Xg(a-) erythrocytes. In antibody-specific immobilization of antigen (ASIA) and immunoblot assays, the anti-peptide antibodies react with the same molecule as does human anti-Xga. Therefore, by its identity with PBDX, Xga is identified as a cell-surface protein that is 48% homologous to CD99 (previously designated the 12E7 antigen), the product of MIC2 which is tightly linked to XG. PBDX is renamed here XG.
我们通过使用针对先前称为PBDX的候选基因N端结构域衍生肽产生的兔多克隆抗体和小鼠单克隆抗体，鉴定了由XG血型基因编码的Xga抗原。在间接血凝试验中，这些抗肽抗体与Xg(a+)红细胞反应，而不与Xg(a-)红细胞反应。在抗原特异性抗体固定（ASIA）和免疫印迹试验中，抗肽抗体与人类抗Xga抗体反应的是同一分子。因此，通过与PBDX的一致性，Xga被鉴定为一种细胞表面蛋白，与CD99（先前称为12E7抗原）有48%的同源性，CD99是与XG紧密连锁的MIC2的产物。PBDX在此重新命名为XG。
