Orfäo A, García-Sanz R, López-Berges M C, Belén Vidriales M, González M, Caballero M D, San Miguel J F
Servicio General de Citometría de Flujo, Universidad de Salamanca, Spain.
Cytometry. 1994 Dec 1;17(4):332-9. doi: 10.1002/cyto.990170409.
In the present paper a CD38/propidium iodide double staining technique is described which separately assesses the cell cycle distribution of myelomatous plasma cells from that of the residual normal hemopoietic cells. For this purpose, bone marrow (BM) cells from a group of 42 untreated multiple myeloma patients were analyzed. Of these, 23 cases were aneuploid (55%) and 19 diploid (45%). The use of the CD38/propidium iodide double staining method allowed a clear separation between CD38 strong positive cells from the remaining bone marrow populations, cell sorting experiments confirming that plasma cells were almost exclusively contained in the former fraction where they represented 97 +/- 2% of the total cells sorted. In all cases, the S-phase in plasma cells and in the remaining normal hemopoietic bone marrow cells was assessed, being higher in normal hemopoietic cells (8.0 +/- 6.3%) than in plasma cells (3.3 +/- 2.6%, P < 0.002). In addition, there was no correlation between the S-phase of the neoplastic and normal bone marrow cells (r = 0.22; P > 0.10); this work therefore shows that the assessment of the total proliferative rate of bone marrow samples does not reflect either the proliferation of normal cells or that of neoplastic plasma cells but will depend on the proliferative rate and the percentage of each population within the sample, which can be assessed by the technique described here.
在本文中，描述了一种CD38/碘化丙啶双重染色技术，该技术可分别评估骨髓瘤浆细胞与残余正常造血细胞的细胞周期分布。为此，对一组42例未经治疗的多发性骨髓瘤患者的骨髓（BM）细胞进行了分析。其中，23例为非整倍体（55%），19例为二倍体（45%）。使用CD38/碘化丙啶双重染色方法能够将CD38强阳性细胞与其余骨髓细胞群清晰分离，细胞分选实验证实浆细胞几乎完全包含在前一组分中，在分选的总细胞中占97±2%。在所有病例中，均评估了浆细胞和其余正常造血骨髓细胞中的S期，正常造血细胞中的S期（8.0±6.3%）高于浆细胞（3.3±2.6%，P<0.002）。此外，肿瘤性和正常骨髓细胞的S期之间无相关性（r = 0.22；P>0.10）；因此，这项研究表明，对骨髓样本总增殖率的评估既不能反映正常细胞的增殖情况，也不能反映肿瘤性浆细胞的增殖情况，而是取决于样本中每个群体的增殖率和百分比，这可以通过本文所述技术进行评估。
