Cascino I, Fiucci G, Papoff G, Ruberti G
Department of Immunobiology, Institute of Cell Biology, National Research Council, Rome, Italy.
J Immunol. 1995 Mar 15;154(6):2706-13.
Fas/Apo-1 molecule is an apoptosis-signaling cell surface Ag belonging to the TNFR family. To investigate the possibility that soluble forms of the Fas receptor are expressed in human cells, we analyzed Fas mRNA transcripts obtained from activated peripheral mononuclear cells of healthy donors and from human tumor cell lines. We identified and characterized three human mRNA Fas variants: FasTMDel, FasDel2, and FasDel3. To determine whether the three transcripts were derived by alternative splicing, the Fas genomic intron/exon organization of the regions surrounding the deleted sequences was analyzed in Fas clones isolated from a human genomic library. Expression of the transcripts was studied in COS cells transiently transfected with the FasTMDel, FasDel2, and FasDel3 cDNAs. Immunocytochemical and in vitro apoptosis inhibition studies suggest that the transcripts are expressed as soluble Fas proteins that may play a functional role in the regulation of apoptosis.
Fas/Apo-1分子是一种属于肿瘤坏死因子受体（TNFR）家族的凋亡信号细胞表面抗原。为了研究可溶性形式的Fas受体在人类细胞中表达的可能性，我们分析了从健康供体活化的外周血单个核细胞和人类肿瘤细胞系中获得的Fas mRNA转录本。我们鉴定并表征了三种人类mRNA Fas变体：FasTMDel、FasDel2和FasDel3。为了确定这三种转录本是否通过可变剪接产生，我们在从人类基因组文库中分离出的Fas克隆中分析了缺失序列周围区域的Fas基因组内含子/外显子组织。在用FasTMDel、FasDel2和FasDel3 cDNA瞬时转染的COS细胞中研究了转录本的表达。免疫细胞化学和体外凋亡抑制研究表明，这些转录本表达为可溶性Fas蛋白，可能在细胞凋亡调节中发挥功能作用。
