Wang X H, Hsieh J T
Guangzhou General Hospital of PLA.
Shi Yan Sheng Wu Xue Bao. 1994 Dec;27(4):447-55.
The effects of androgen on cytokeratin (CK) 8 mRNA expression in rat ventral prostate (VP) were studied by in situ hybridization with an antisense RNA-probe. It was found that 1) the CK 8 antisense RNA-probe was accumulated only within prostatic epithelial cells, indicating that the CK 8 mRNA is a specific and sensitive marker for prostate epithelia. 2) After castration, the signals of CK 8 mRNA in VP sections were significantly increased. Administration of androgen to the castrated male host repressed the elevated expression of CK 8 mRNA in VP. This effect can be antagonized by the simultaneous administration of an antiandrogen. 3) Unlike other androgen-repressed gene, the elevated expression of CK 8 mRNA in VP was persisted even 2 months after the process of glandular involution was completed. 4) During prostate development, the strongest CK 8 mRNA staining was found in the early neonatal prostatic epithelia which were composed mainly of prostatic stem cells. Thereafter, when the levels of serum androgen were gradually increased, the shift of CK 8 mRNA staining to peripheral region and decreased level of overall CK 8 mRNA were noted. These data support the notion that the CK 8 gene of prostate epithelia is a new class of androgen-repressed one. The data also indicated that the expression of CK 8 gene is closely related with the proliferation and differentiation of prostate stem cells, and excessive expression of CK 8 mRNA is a characteristic for prostatic stem cells.
采用反义RNA探针原位杂交技术，研究雄激素对大鼠前列腺腹侧叶（VP）细胞角蛋白（CK）8 mRNA表达的影响。结果发现：1）CK 8反义RNA探针仅在前列腺上皮细胞内积聚，表明CK 8 mRNA是前列腺上皮细胞特异性及敏感性的标志物。2）去势后，VP切片中CK 8 mRNA信号显著增强。给去势雄鼠注射雄激素可抑制VP中CK 8 mRNA的升高表达。此效应可被同时注射抗雄激素所拮抗。3）与其他雄激素抑制基因不同，即使在腺体退化过程完成2个月后，VP中CK 8 mRNA的升高表达仍持续存在。4）在前列腺发育过程中，在主要由前列腺干细胞组成的新生早期前列腺上皮中发现最强的CK 8 mRNA染色。此后，随着血清雄激素水平逐渐升高，可见CK 8 mRNA染色向周边区域转移，且CK 8 mRNA总体水平降低。这些数据支持前列腺上皮CK 8基因是一类新的雄激素抑制基因的观点。数据还表明，CK 8基因的表达与前列腺干细胞的增殖和分化密切相关，CK 8 mRNA的过度表达是前列腺干细胞的一个特征。
