Larquet E, Boisset N, Pochon F, Lamy J
Laboratoire de Microscopie Cellulaire et Moléculaire, CNRS URA 147, Institut Gustave-Roussy, Villejuif, France.
J Struct Biol. 1994 Jul-Aug;113(1):87-98. doi: 10.1006/jsbi.1994.1034.
The architecture of the native human alpha 2-macroglobulin was studied by cryoelectron microscopy and image processing techniques. The lip, padlock, doughnut, and four-petaled flower views of this homotetrameric proteinase inhibitor were observed in the frozen-hydrated specimen, and a new view, termed eye view, was also characterized. The present three-dimensional reconstruction demonstrates that all these electron microscope views derive from a single three-dimensional structure. The molecule is composed of two horizontal bodies and of two oblique arches, which border a large central cavity. The polymorphism and the flexibility of the native alpha 2-macroglobulin are discussed.
通过冷冻电子显微镜和图像处理技术研究了天然人α2-巨球蛋白的结构。在冷冻水合标本中观察到这种同四聚体蛋白酶抑制剂的唇部、锁扣、甜甜圈和四瓣花视图，还表征了一种新的视图，称为眼视图。目前的三维重建表明，所有这些电子显微镜视图都源自单一的三维结构。该分子由两个水平体和两个倾斜拱组成，它们围绕着一个大的中央腔。讨论了天然α2-巨球蛋白的多态性和灵活性。
