Reynolds G M, Young F I, Young J A, Williams A, Rowlands D C
Department of Pathology, University of Birmingham, UK.
Cytopathology. 1994 Dec;5(6):345-58. doi: 10.1111/j.1365-2303.1994.tb00440.x.
Microwave oven antigen retrieval has been developed to extend the range of antibodies that can be used upon sections of fixed and processed tissue. It has the additional advantages of improving immunostain intensity and reducing background positivity. It can also be employed as an alternative to proteolytic digestion. In this study the effects of microwave oven heating upon immunochemical staining of cytopathological specimens with a range of selected antibodies have been investigated. Microwaving did not result in loss of cells, and there was no need to use adhesive-coated slides. The method improved the staining intensity and reduced background with antibodies against a variety of antigens that are difficult or impossible to detect in formaldehyde-fixed cytological material. Microwave heating was also used successfully as an alternative to trypsin digestion, and had the advantage of reduced morphological distortion. The technique was useful in demonstrating the soluble formalin-sensitive antigen p19 on cytospins fixed in formaldehyde vapour. Microwave oven heating thus shows promise of extending the scope of immunostaining in clinical cytopathology.
已开发出微波炉抗原修复技术，以扩大可用于固定和处理过的组织切片的抗体范围。它还具有提高免疫染色强度和降低背景阳性率的额外优势。它也可作为蛋白水解消化的替代方法。在本研究中，已研究了微波炉加热对一系列选定抗体对细胞病理学标本进行免疫化学染色的影响。微波处理不会导致细胞丢失，也无需使用涂有粘合剂的载玻片。该方法提高了针对多种抗原的抗体的染色强度并降低了背景，这些抗原在甲醛固定的细胞学材料中难以或无法检测到。微波加热也成功用作胰蛋白酶消化的替代方法，并且具有减少形态学畸变的优点。该技术有助于在甲醛蒸汽固定的细胞涂片上显示可溶性福尔马林敏感抗原p19。因此，微波炉加热有望扩大临床细胞病理学中免疫染色的范围。
