Nitric oxide-synthesizing neurons in the hamster suprachiasmatic nucleus: a combined NOS- and NADPH- staining and retinohypothalamic tract tracing study.

Neuronal nitric oxide (NO), thought to be a neuroactive substance of high potency, is produced by the enzyme nitric oxide synthase (NOS) which has been demonstrated to additionally exhibit a so-called NADPH-diaphorase (NADPH-d) activity. Since physiological results pointed to the involvement of NO in circadian regulation, and morphological descriptions are not available, we sought to study the distribution of NO-producing cells in the hypothalamic suprachiasmatic nucleus (SCN) in Djungarian hamsters (Phodopus sungorus) by means of histochemistry and immunohistochemistry (IHC). In the SCN, NADPH-d stained perikarya of varying intensity and number were found predominantly in the ventrolateral subdivision. Diaphorase staining combined with the IHC demonstration of NOS revealed a complete overlapping of both. The combination of NADPH-d staining with the demonstration of the retinohypothalamic tract using the anterograde neuronal transport of cholera toxin B (CTB) following intraocular injection showed CTB terminals accumulating at NADPH-d cell bodies mainly in the ventrolateral region of the SCN. These data provide morphological evidence for the involvement of nitric oxide in the mediation of photic stimulation of the circadian oscillator located in the SCN.