Morphological evidence for the presence of ipsilateral inferior olivary neurons during postnatal development of the olivocerebellar projection in the rat.

The presence of ipsilateral inferior olivary neurons during postnatal development of the olivocerebellar projection in the rat was investigated by two in vitro axonal tracing methods and by the axotomy of one olivocerebellar tract. The experiments were carried out before (P1), during (P5-P10) and after (P20) the period of multiple innervation of Purkinje cells by climbing fibers. According to present results: (1) ipsilateral inferior olivary neurons are distributed, on all analyzed days, throughout the entire inferior olive; (2) cell counts after axotomy experiments demonstrated that they represent a small population of inferior olivary neurons, whose number oscillated between 271 +/- 30 in young animals (pedunculotomized at P1 and killed at P7) and 26 +/- 12 in older ones (pedunculotomized at P20 and killed at P40). This experiment confirmed that most of these neurons are eliminated during the regressive events that take place during normal development of the olivocerebellar projection; and (3) few ipsilateral inferior olivary neurons, however, survive at P40, but their significance is still unclear.