López-Román A, Armengol J A
Departamento de Ciencias Morfológicas, Facultad de Medicina, Universidad de Sevilla, Spain.
J Comp Neurol. 1994 Dec 15;350(3):485-96. doi: 10.1002/cne.903500312.
The presence of ipsilateral inferior olivary neurons during postnatal development of the olivocerebellar projection in the rat was investigated by two in vitro axonal tracing methods and by the axotomy of one olivocerebellar tract. The experiments were carried out before (P1), during (P5-P10) and after (P20) the period of multiple innervation of Purkinje cells by climbing fibers. According to present results: (1) ipsilateral inferior olivary neurons are distributed, on all analyzed days, throughout the entire inferior olive; (2) cell counts after axotomy experiments demonstrated that they represent a small population of inferior olivary neurons, whose number oscillated between 271 +/- 30 in young animals (pedunculotomized at P1 and killed at P7) and 26 +/- 12 in older ones (pedunculotomized at P20 and killed at P40). This experiment confirmed that most of these neurons are eliminated during the regressive events that take place during normal development of the olivocerebellar projection; and (3) few ipsilateral inferior olivary neurons, however, survive at P40, but their significance is still unclear.
通过两种体外轴突追踪方法以及切断一条橄榄小脑束，研究了大鼠橄榄小脑投射在出生后发育过程中同侧下橄榄核神经元的存在情况。实验在浦肯野细胞被攀缘纤维多重支配之前（P1）、期间（P5 - P10）和之后（P20）进行。根据目前的结果：（1）在所有分析的天数中，同侧下橄榄核神经元分布于整个下橄榄核；（2）切断轴突实验后的细胞计数表明，它们代表了一小部分下橄榄核神经元，其数量在幼龄动物（P1进行脑桥切断并在P7处死）中为271±30，在老龄动物（P20进行脑桥切断并在P40处死）中为26±12之间波动。该实验证实，这些神经元中的大多数在橄榄小脑投射正常发育过程中发生的退行性事件中被消除；（3）然而，少数同侧下橄榄核神经元在P40存活，但其意义仍不清楚。
