Höfler H, Mueller J
Institut für Allgemeine Pathologie und Pathologische Anatomie, Technischen Universität München.
Verh Dtsch Ges Pathol. 1994;78:124-35.
The technique of in situ hybridization (ISH) is presented with special reference to its use in the practice of pathology. Methodological and theoretical aspects of ISH are first considered with regard to tissue fixation, probe selection and preparation, various types of labeling reactions and aspects of hybridization conditions as they relate to hybridization protocols. This is followed by a discussion about the interpretation of the results of ISH with regard to sensitivity and specificity and the importance of, and various types of, controls. Lastly, specific applications of ISH in pathology are discussed, concluding with possible future applications for ISH in research and diagnosis.
本文介绍了原位杂交（ISH）技术，并特别提及了其在病理学实践中的应用。首先从组织固定、探针选择与制备、各类标记反应以及与杂交方案相关的杂交条件等方面，探讨了ISH的方法学和理论问题。接着讨论了ISH结果在敏感性和特异性方面的解读，以及对照的重要性和种类。最后讨论了ISH在病理学中的具体应用，并对ISH在未来研究和诊断中的可能应用进行了总结。
