Schlegel J, Scherthan H, Arens N, Stumm G, Cremer T, Kiessling M
Institut für Pathologie, Universität Regensburg.
Verh Dtsch Ges Pathol. 1994;78:204-7.
Comparative genomic hybridization (CGH) provides a new possibility for the investigation of genetic alterations in tumour genomes. In our experiments CGH was carried out using genomic DNA from human glioblastoma multiforme (GBM) as a probe for chromosomal in situ suppression hybridization. Amplified DNA sequences contained in the tumour DNA showed specific signals, revealing the chromosomal positions of these sequences. Using this approach we detected amplifications of different chromosomal segments in individual GBM specimens. In accordance with the results from Southern analysis demonstrating amplification of the EGFR gene in 45% of human GBM, CGH signals in different GBM mapped to the region of this gene on chromosome 7p. Other signals detected by CGH involved chromosome 12q and 8q. Our data demonstrate CGH as a novel comprehensive and rapid approach for the analysis of complex genomic alterations in glial tumours.
比较基因组杂交（CGH）为研究肿瘤基因组中的基因改变提供了新的可能性。在我们的实验中，使用来自多形性胶质母细胞瘤（GBM）的基因组DNA作为染色体原位抑制杂交的探针进行CGH。肿瘤DNA中包含的扩增DNA序列显示出特定信号，揭示了这些序列的染色体位置。使用这种方法，我们在单个GBM标本中检测到不同染色体片段的扩增。根据Southern分析结果显示45%的人类GBM中存在表皮生长因子受体（EGFR）基因扩增，不同GBM中的CGH信号定位于7号染色体p臂上该基因的区域。CGH检测到的其他信号涉及12号染色体q臂和8号染色体q臂。我们的数据表明CGH是一种用于分析胶质肿瘤中复杂基因组改变的新型综合快速方法。
