Barten M, Ostwald C, Müller P, Löning T, Milde-Langosch K, Wukasch Y
Institut für Pathologie, Universität Rostock.
Verh Dtsch Ges Pathol. 1994;78:255-9.
Immunohistochemical examination of p53 protein accumulation, analysis of the p53 gene using amplification of the exons 5-8 followed by TGGE, and PCR based HPV typing were carried out on 40 oral squamous cell carcinomas. 28 of 40 oral carcinomas (70%) contained DNA of the oncogenic HPV types 16 and 18. A p53 accumulation was present in 20 of 37 tumors (54%). Mutations of the p53 gene were detected in 15 of 39 carcinomas (38%). 15 of 20 oral carcinomas with abnormal p53 expression and 11/15 tumors with mutations of the p53 gene were HPV positive. Our results demonstrate a frequent occurrence of p53 abnormalities in HPV positive oral squamous cell carcinomas. This pattern is divergent from HPV related genital carcinomas and could reflect differences in the etiology of squamous cell carcinomas of different sites.
对40例口腔鳞状细胞癌进行了p53蛋白积累的免疫组织化学检查、采用外显子5 - 8扩增后行TGGE分析p53基因以及基于PCR的HPV分型。40例口腔癌中有28例（70%）含有致癌性HPV 16型和18型的DNA。37例肿瘤中有20例（54%）存在p53积累。39例癌中有15例（38%）检测到p53基因突变。20例p53表达异常的口腔癌中有15例以及15例p53基因突变的肿瘤中有11例为HPV阳性。我们的结果表明，HPV阳性的口腔鳞状细胞癌中p53异常情况频繁发生。这种模式与HPV相关的生殖器癌不同，可能反映了不同部位鳞状细胞癌病因的差异。
