Huettner C, Paulus W, Roggendorf W
Institut für Pathologie, Universität Würzburg.
Verh Dtsch Ges Pathol. 1994;78:418-22.
Interleukin 10 (Il-10) was initially discovered on the basis of its ability to suppress cytokine synthesis. Additionally, it can exert immunosuppressive effects on a variety of cell types. Since patients with malignant gliomas present with a general impairment of the immune system, we sought to investigate if IL-10 is expressed in the glioma tissue. Using RT-PCR, IL-10 mRNA levels were determined in 37 glial tumors of different grades including 2 recurrencies, 3 specimens from normal brain tissue and 3 glioblastoma cell lines. Expression of IL-10 mRNA was demonstrable in all tumors as well as in normal brain. High grade tumors and recurrent cases expressed significantly higher amounts of IL-10 specific mRNA compared to low grade tumors, while 2 out of 3 cell lines showed only weak constitutive expression. We suggest, that IL-10 may contribute to the progression of astrocytomas by allowing the tumor cells to attenuate the T-cell immune response and evade immune detection.
白细胞介素10（IL-10）最初是基于其抑制细胞因子合成的能力而被发现的。此外，它可对多种细胞类型发挥免疫抑制作用。由于恶性胶质瘤患者存在免疫系统的普遍损害，我们试图研究IL-10是否在胶质瘤组织中表达。使用逆转录聚合酶链反应（RT-PCR），在37个不同级别的胶质肿瘤（包括2例复发病例）、3个正常脑组织标本和3个胶质母细胞瘤细胞系中测定了IL-10信使核糖核酸（mRNA）水平。IL-10 mRNA在所有肿瘤以及正常脑组织中均有表达。与低级别肿瘤相比，高级别肿瘤和复发病例中IL-10特异性mRNA的表达量显著更高，而3个细胞系中有2个仅显示出微弱的组成性表达。我们认为，IL-10可能通过使肿瘤细胞减弱T细胞免疫反应并逃避免疫检测，从而促进星形细胞瘤的进展。
