Vigne J L, Halburnt L L, Skinner M K
Reproductive Endocrinology Center, University of California, San Francisco 94143-0556.
Biol Reprod. 1994 Dec;51(6):1213-21. doi: 10.1095/biolreprod51.6.1213.
The majority of ovarian cancers are derived from a single layer of epithelial cells that covers the surface of the ovary termed the ovarian surface epithelium (OSE). Ovarian surface stromal cells underlie the OSE and have a morphology distinct from that of the interstitial stromal cells between follicles. Because of the similarities between bovine and human ovarian physiology, and to allow an adequate supply of tissue and cells, bovine OSE and stromal cell cultures were established to investigate the cell biology of these cell types. Morphological analysis of bovine ovarian sections revealed that several layers of ovarian surface stromal cells can be identified and are structurally distinct from interstitial stromal cells. Both OSE and stromal cells can be isolated and cultured for weeks in the absence of presence of serum. The cell populations were found, through use of a keratin immunocytochemical stain for OSE, to be highly purified. To investigate the functional properties of the two cell types, radiolabeled secreted proteins were collected and electrophoretically analyzed. The radiolabeled secreted protein profiles of OSE and stromal cells were found to be distinct with a discrete number of secreted proteins. Major OSE secretory products were obtained from serum-free concentrated conditioned medium, electrophoretically separated, blotted, and sequenced. Two OSE secretory products of 28 kDa and 40 kDa were sequenced and found to match a sequence in the computerized database. The 28-kDa OSE protein was identified as a tissue inhibitor of metalloproteinase, TIMP. The 40-kDa OSE protein was identified as the insulin-like growth factor (IGF) binding protein-2 (IGFBP2).(ABSTRACT TRUNCATED AT 250 WORDS)
大多数卵巢癌起源于覆盖卵巢表面的单层上皮细胞，即卵巢表面上皮（OSE）。OSE下方是卵巢表面基质细胞，其形态与卵泡间的间质基质细胞不同。由于牛和人类卵巢生理学存在相似性，且为了获得充足的组织和细胞供应，建立了牛OSE和基质细胞培养体系，以研究这些细胞类型的细胞生物学。对牛卵巢切片的形态学分析显示，可以识别出几层卵巢表面基质细胞，其结构与间质基质细胞不同。OSE和基质细胞均可在有无血清的情况下分离培养数周。通过使用针对OSE的角蛋白免疫细胞化学染色发现，细胞群体高度纯化。为了研究这两种细胞类型的功能特性，收集了放射性标记的分泌蛋白并进行电泳分析。发现OSE和基质细胞的放射性标记分泌蛋白谱不同，有数量不等的分泌蛋白。主要的OSE分泌产物从无血清浓缩条件培养基中获得，经电泳分离、印迹和测序。对两种分子量分别为28 kDa和40 kDa的OSE分泌产物进行了测序，发现它们与计算机数据库中的一个序列匹配。28 kDa的OSE蛋白被鉴定为金属蛋白酶组织抑制剂（TIMP）。40 kDa的OSE蛋白被鉴定为胰岛素样生长因子（IGF）结合蛋白-2（IGFBP2）。（摘要截短于250字）
