Guichard G, Benkirane N, Graff R, Muller S, Briand J P
Institut de Biologie Moléculaire et Cellulaire, UPR 9021 CNRS, Strasbourg, France.
Pept Res. 1994 Nov-Dec;7(6):308-21.
Pseudopeptide analogues of the C-terminal hexapeptide of histone H3 (-Ile-Arg-Gly-Glu-Arg-Ala-OH) were obtained by systematically replacing, in each analogue, one peptide bond at a time by a reduced peptide bond psi (CH2-NH). The resulting analogues were then examined, in ELISA and in the BIAcore system, for their ability to bind polyclonal and monoclonal antibodies generated against the parent natural peptide and the protein. The comparative results show that reduced bond pseudopeptide analogues can mimic the parent peptide. These results present the first unequivocal example for the potent applicability of reduced peptide bond pseudopeptides in the immunological field.
通过在每个类似物中每次用还原肽键psi（CH2-NH）系统地替换一个肽键，获得了组蛋白H3 C末端六肽（-Ile-Arg-Gly-Glu-Arg-Ala-OH）的假肽类似物。然后在ELISA和BIAcore系统中检测所得类似物与针对亲本天然肽和蛋白质产生的多克隆和单克隆抗体结合的能力。比较结果表明，还原键假肽类似物可以模拟亲本肽。这些结果为还原肽键假肽在免疫学领域的有效应用提供了第一个明确的例子。
