AMeX方法在抗角蛋白抗体免疫染色中的应用价值。
Usefulness of the AMeX method for immunostaining with antikeratin antibodies.
作者信息
Ansai S I, Koseki S, Hozumi Y, Kondo S
机构信息
Department of Dermatology, Yamagata University School of Medicine, Japan.
出版信息
Clin Exp Dermatol. 1994 Nov;19(6):458-62. doi: 10.1111/j.1365-2230.1994.tb01247.x.
We performed an immunohistochemical study with 11 antikeratin antibodies using the newly developed AMeX (acetone-methylbenzoate-xylene) tissue processing method. Specimens processed with this method showed almost as good preservation and morphological detail as routinely processed, formalin-fixed and paraffin-embedded tissue specimens, and as good preservation of antigenicity as fresh frozen tissue specimens. Thus, we propose the wide use of this method in dermatology.
我们使用新开发的AMeX（丙酮-甲基苯甲酸酯-二甲苯）组织处理方法，用11种抗角蛋白抗体进行了免疫组织化学研究。用这种方法处理的标本显示出与常规处理的福尔马林固定石蜡包埋组织标本几乎一样好的保存效果和形态细节，以及与新鲜冷冻组织标本一样好的抗原性保存效果。因此，我们建议在皮肤病学中广泛使用这种方法。
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