Dominskiĭ D A, Pokrovskaia E S, Babushkina E A, Turkina A G, Grineva N I
Genetika. 1994 Dec;30(12):1636-9.
A modified system with two pairs of primers is suggested for the analysis of different bcr/abl mRNA variants, using the polymerase chain reaction (PCR) method. It was demonstrated that, with the use of this method, mRNA preparations obtained from bone marrow cells are more informative than those obtained from blood cells. Fresh preparations of thermostable Tth DNA polymerase, which exhibited the reverse transcriptase activity, appeared to be optimal for PCR analysis of the bcr/abl mRNA pattern.
建议使用一种改良的系统，该系统有两对引物，采用聚合酶链反应（PCR）方法分析不同的bcr/abl mRNA变体。结果表明，使用这种方法，从骨髓细胞获得的mRNA制剂比从血细胞获得的更具信息性。表现出逆转录酶活性的热稳定Tth DNA聚合酶新鲜制剂似乎是用于bcr/abl mRNA模式PCR分析的最佳选择。
