Bergsagel P L, Masellis Smith A, Belch A R, Pilarski L M
NCI-Navy Medical Oncology Branch, National Cancer Institute, Bethesda, MD 20889-5015.
Curr Top Microbiol Immunol. 1995;194:17-24. doi: 10.1007/978-3-642-79275-5_3.
Previous reports have described the phenotypic and functional properties of monotypic late stage B cells in the blood of patients with multiple myeloma and have speculated that these B cells represent a malignant circulating component of myeloma. Here we show that blood B cells have IgH rearrangements identical to those expressed by the bone marrow plasma cells by using Ig Fingerprint and Allele-Specific Oligomer (ASO) polymerase chain reaction (PCR) methods. DNA from purified blood B cells and bone marrow plasma cells taken at the same time, and blood B cells taken at subsequent patient visits was amplified using consensus IgH primers, or ASO primers. In 10/16 patients, a single IgH rearrangement was amplified from the bone marrow plasma cells. In all 10 of those patients the same clonotypic rearrangement was amplified from the purified blood B cells. The relationship of these clonal blood B cells to the malignant bone marrow plasma cells remains undetermined.
先前的报告描述了多发性骨髓瘤患者血液中单一型晚期B细胞的表型和功能特性，并推测这些B细胞代表骨髓瘤的恶性循环成分。在此我们使用Ig指纹和等位基因特异性寡聚物（ASO）聚合酶链反应（PCR）方法表明，血液B细胞具有与骨髓浆细胞所表达的相同的IgH重排。使用共有IgH引物或ASO引物对同时采集的纯化血液B细胞和骨髓浆细胞以及患者后续就诊时采集的血液B细胞的DNA进行扩增。在16例患者中的10例中，从骨髓浆细胞中扩增出单一的IgH重排。在所有这10例患者中，从纯化的血液B细胞中扩增出相同的克隆型重排。这些克隆性血液B细胞与恶性骨髓浆细胞之间的关系仍未确定。
