苯丙氨酸-蛋氨酸-精氨酸-苯丙氨酸-氨基（FMRFa）相关肽对完整大鼠胰腺β细胞中钠/钙交换的抑制作用。

Inhibition of Na/Ca exchange by Phe-Met-Arg-Phe-NH2 (FMRFa)-related peptides in intact rat pancreatic B-cells.

作者信息

Van Eylen F, Gourlet P, Vandermeers A, Lebrun P, Herchuelz A

机构信息

Laboratory of Pharmacology, Brussels University School of Medicine, Belgium.

出版信息

Mol Cell Endocrinol. 1994 Dec;106(1-2):R1-5. doi: 10.1016/0303-7207(94)90209-7.

DOI:10.1016/0303-7207(94)90209-7

PMID:7534729

Abstract

Phe-Met-Arg-Phe-NH2 (FMRFa)-related peptides were recently shown to inhibit Na/Ca exchange in cardiac sarcolemmal vesicles. In the present study, we examined the effects of FMRFa-related peptides on Na/Ca exchange in intact (pancreatic B) cells. At 2.8 mM glucose, FMRFa-related peptides only weakly inhibited Na/Ca exchange although their effect was more marked under depolarizing conditions. The peptides blocked neither the Na/K-ATPase nor Ca2+ channels but slightly reduced membrane K+ permeability. Our data indicate that FMRFa-related peptides are weak and non-specific inhibitors of Na/Ca exchange in intact B cells. The data do not confirm the view that the peptides may exert some of their physiological modulatory role by inhibiting Na/Ca exchange.

摘要

苯丙氨酸-甲硫氨酸-精氨酸-苯丙氨酸-氨基（FMRFa）相关肽最近被证明可抑制心肌肌膜囊泡中的钠/钙交换。在本研究中，我们研究了FMRFa相关肽对完整（胰腺B）细胞中钠/钙交换的影响。在2.8 mM葡萄糖浓度下，FMRFa相关肽仅微弱抑制钠/钙交换，尽管在去极化条件下其作用更为明显。这些肽既不阻断钠/钾-ATP酶也不阻断钙通道，但会轻微降低膜钾通透性。我们的数据表明，FMRFa相关肽是完整B细胞中钠/钙交换的弱且非特异性抑制剂。这些数据并未证实该肽可能通过抑制钠/钙交换发挥其某些生理调节作用的观点。