von Poblotzki A, Gigler A, Lang B, Wolf H, Modrow S
Institut für medizinische Mikrobiologie und Hygiene, Universität Regensburg, Germany.
J Gen Virol. 1995 Mar;76 ( Pt 3):519-27. doi: 10.1099/0022-1317-76-3-519.
Human parvovirus B19 is the aetiological agent of the common childhood disease erythema infectiosum (fifth disease). The infection is usually benign and self-limiting, but in adults cases of severe arthritis which may persist for years have been reported. Neutralizing antibodies directed against the structural proteins are usually produced shortly after the infection. The immune response against the third major protein, the non-structural protein NS-1, of parvovirus B19 has not been characterized so far. We cloned and expressed the full-length NS-1 protein and fragments thereof in Escherichia coli. The purified recombinant proteins were used to investigate the presence of antibodies to the NS-1 protein in sera from patients with parvovirus B19 infection. Specific antibodies could be detected in sera from patients suffering from severe parvovirus B19-associated arthritis using Western blot analysis and an ELISA. Sera from patients with acute or past infection without complications did not contain detectable levels of immunoglobulin to NS-1. The use of subfragments of the NS-1 protein allowed localization of the antigenic domains in the carboxy-terminal region of the protein.
人细小病毒B19是常见儿童疾病传染性红斑（第五病）的病原体。该感染通常为良性且具有自限性，但在成人中，有报告称会出现可能持续数年的严重关节炎病例。针对结构蛋白的中和抗体通常在感染后不久产生。迄今为止，针对细小病毒B19的第三种主要蛋白——非结构蛋白NS-1的免疫反应尚未得到明确描述。我们在大肠杆菌中克隆并表达了全长NS-1蛋白及其片段。纯化后的重组蛋白用于研究细小病毒B19感染患者血清中针对NS-1蛋白的抗体抗体的存在情况。使用蛋白质印迹分析和酶联免疫吸附测定法可在患有严重细小病毒B19相关关节炎的患者血清中检测到特异性抗体。急性感染或既往感染且无并发症的患者血清中未检测到可检测水平的针对NS-1的免疫球蛋白。使用NS-1蛋白的亚片段可确定该蛋白羧基末端区域的抗原结构域。
