Rubbi C P, Qiu J, Rickwood D
Department of Biology, University of Essex, Wivenhoe Park, Colchester, U.K.
Eur J Histochem. 1994;38(4):269-80.
A variety of compounds which are known to cross-link proteins have been tested as possible cell fixatives for confocal microscopy. The criteria for good fixation that were used for this work were that the fixative should make the cells resistant to lysis and the loss of protein from the cell under hypotonic conditions. In addition, fixation should not change the shape nor the volume of the cell. Of the compounds tested, one compound, EDC, appeared to have an excellent potential as a cell fixative and has the additional advantage that it does not require amino groups for its crosslinking activity. Another compound, SPDP, appeared to have some potential as a readily reversible fixative by virtue of its disulfide bridge.
多种已知能使蛋白质交联的化合物已作为共聚焦显微镜可能的细胞固定剂进行了测试。这项工作所采用的良好固定标准是，固定剂应使细胞在低渗条件下抵抗裂解和蛋白质从细胞中流失。此外，固定不应改变细胞的形状和体积。在所测试的化合物中，一种化合物EDC似乎具有作为细胞固定剂的极佳潜力，并且还有一个额外的优点，即其交联活性不需要氨基。另一种化合物SPDP，由于其二硫键，似乎具有作为一种易于逆转的固定剂的一些潜力。
