Du M H, Spohr U, Lemieux R U
Department of Chemistry, University of Alberta, Edmonton, Canada.
Glycoconj J. 1994 Oct;11(5):443-61. doi: 10.1007/BF00731281.
The chemical mapping of the regions of H-type 2 human blood group-related trisaccharide (Fuc alpha (1-2)Gal beta (1-4)GlcNAc beta Me) that are recognized by three different lectins, the so-called epitopes, are reviewed together with an account of how and why oligosaccharides form specific complexes with proteins as presently viewed in this laboratory. The occasion is used to report the synthesis of the various mono-O-methyl derivatives of the above trisaccharide that were used in these investigations. Also, Fuc alpha (1-2)Gal beta (1-4)Xyl beta Me was synthesized in order to examine whether or not the hydroxymethyl group of the GlcNAc residue participates in the binding reaction.
本文综述了三种不同凝集素所识别的与人类2型血型相关的三糖（Fucα(1-2)Galβ(1-4)GlcNAcβMe）区域的化学图谱，即所谓的表位，并阐述了在本实验室目前的观点中，寡糖如何以及为何与蛋白质形成特定复合物。借此机会报告了在这些研究中使用的上述三糖各种单-O-甲基衍生物的合成。此外，还合成了Fucα(1-2)Galβ(1-4)XylβMe，以研究GlcNAc残基的羟甲基是否参与结合反应。
