Yan L, Wilkins P P, Alvarez-Manilla G, Do S I, Smith D F, Cummings R D
Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA.
Glycoconj J. 1997 Jan;14(1):45-55. doi: 10.1023/a:1018508914551.
A defined set of oligosaccharides and glycopeptides containing alpha-linked fucose were used to examine the specificity of the immobilized fucose-binding lectin Lotus tetragonolobus agglutinin (LTA1), also known as lotus lectin. Glycans containing the Lewis x determinant (Le(x)) Gal beta 1-4[Fuc alpha 1-3]GlcNAc beta 1-3-R were significantly retarded in elution from high density LTA-Emphaze columns. The lectin also bound the fucosylated lacdiNAc trisaccharide GalNAc beta 1-4[Fuc alpha 1-3]GlcNAc. The lectin did not bind glycans containing either sialylLe(x) or VIM-2 determinants, nor did it bind the isomeric Le(x), Gal beta 1-3[Fuc alpha 1-4]GlcNAc-R. Although 2'-fucosyllactose Fuc alpha 1-2Gal beta 1-4Glc) was retarded in elution from the columns, larger glycans containing the H-antigen Fuc alpha 1-2Gal beta 1-3(4)GlcNAc-R interacted poorly with immobilized LTA. Our results demonstrate that immobilized LTA is effective in isolating glycans containing the Le(x) antigen and is useful in analyzing specific fucosylation of glycoconjugates.
使用一组特定的含有α-连接岩藻糖的寡糖和糖肽来检测固定化的岩藻糖结合凝集素四角豆凝集素（LTA1，也称为莲凝集素）的特异性。含有路易斯x决定簇（Le(x)）Galβ1-4[Fucα1-3]GlcNAcβ1-3-R的聚糖从高密度LTA-Emphaze柱上洗脱时明显延迟。该凝集素还结合岩藻糖基化的乳糖二胺三糖GalNAcβ1-4[Fucα1-3]GlcNAc。该凝集素不结合含有唾液酸化路易斯x或VIM-2决定簇的聚糖，也不结合异构体Le(x) Galβ1-3[Fucα1-4]GlcNAc-R。虽然2'-岩藻糖基乳糖（Fucα1-2Galβ1-4Glc）从柱上洗脱时延迟，但含有H抗原Fucα1-2Galβ1-3(4)GlcNAc-R的较大聚糖与固定化LTA的相互作用较差。我们的结果表明，固定化LTA可有效分离含有Le(x)抗原的聚糖，可用于分析糖缀合物的特定岩藻糖基化。
