引物tRNA（Lys3）与人免疫缺陷病毒1型逆转录酶p51亚基的相互作用：在酶激活中的可能作用。

Interaction of primer tRNA(Lys3) with the p51 subunit of human immunodeficiency virus type 1 reverse transcriptase: a possible role in enzyme activation.

作者信息

Zakharova O D, Tarrago-Litvak L, Fournier M, Andreola M L, Repkova M N, Venyaminova A G, Litvak S, Nevinsky G A

机构信息

Institute of Bioorganic Chemistry, Siberian Division of the Academy of Sciences of Russia, Novosibirsk.

出版信息

FEBS Lett. 1995 Mar 20;361(2-3):287-90. doi: 10.1016/0014-5793(95)00200-s.

DOI:10.1016/0014-5793(95)00200-s

PMID:7535248

Abstract

In the interaction between HIV-1 RT and tRNA(Lys3) each subunit of the heterodimer interacts with tRNA showing a different affinity: Kd (p66) = 23 nM, Kd (p51) = 140 nM. Preincubation of heterodimeric RT with tRNA, at concentrations similar to that of the Kd value for p51, leads to an increase of the catalytic activity on poly(A)-oligo(dT). These results were compared to those using different tRNA analogs: oxidized tRNA, tRNAs lacking one, two or three nucleotides from the 3'-end, or ribo- and deoxyribonucleotides mimicking the anticodon loop sequence. In all cases, tRNA analogs were weaker activators of HIV-1 RT than natural tRNA. A possible mechanism of RT p66/p51 activation by tRNA and its analogs, mediated through the p51 subunit, is discussed.

摘要

在HIV-1逆转录酶（RT）与tRNA（Lys3）的相互作用中，异二聚体的每个亚基与tRNA相互作用，显示出不同的亲和力：解离常数（Kd）（p66）= 23 nM，Kd（p51）= 140 nM。用与p51的Kd值相似浓度的tRNA对异二聚体RT进行预孵育，会导致其对聚（A）-寡聚（dT）的催化活性增加。将这些结果与使用不同tRNA类似物的结果进行了比较：氧化型tRNA、3'-末端缺少一个、两个或三个核苷酸的tRNA，或模拟反密码子环序列的核糖核苷酸和脱氧核糖核苷酸。在所有情况下，tRNA类似物作为HIV-1 RT的激活剂都比天然tRNA弱。本文讨论了通过p51亚基介导的tRNA及其类似物激活RT p66/p51的一种可能机制。