Koike D, Yamadera K, DiMagno E P
Gastrointestinal Research Unit, Mayo Clinic, Rochester, Minnesota.
Gastroenterology. 1995 Apr;108(4):1221-9. doi: 10.1016/0016-5085(95)90223-6.
BACKGROUND/AIMS: Chronic amylase inhibition might be useful to treat diabetes mellitus and obesity. Duodenal and ileal cannulas were placed in 8 dogs to determine if long-term ingestion of a wheat amylase inhibitor maintained amylase inhibition or affected gastrointestinal or metabolic function or pancreatic growth.
Five dogs were fed and 3 were not fed 1.5 g of the inhibitor with meals for 9 weeks. Postprandial and cholecystokinin octapeptide stimulated pancreatic secretion, and fecal balance studies were performed at intervals. After the experiment, the pancreas was analyzed.
Weight loss was similar in both groups. Amylase inhibition persisted throughout the 9 weeks; it declined from 91% to 37% from the first to the sixth postprandial hour. Amylase inhibition decreased plasma glucose levels during the first hour (P < 0.05), increased carbohydrate delivery to the ileum (315 vs. 555 mg/h; P = 0.002), and increased cholecystokinin octapeptide-stimulated amylase secretion. However, amylase inhibition did not significantly change plasma concentrations of insulin, peptide YY or neurotensin, postprandial pancreatic secretion, gastrointestinal transit or pancreatic weight, and protein or DNA content.
Prandial ingestion of 1.5 g of the inhibitor for 9 weeks reduces postprandial amylase levels enough to delay carbohydrate digestion and absorption and lower plasma glucose levels without altering pancreatic growth. This dose may be effective to treat diabetes mellitus but not obesity.
背景/目的：长期抑制淀粉酶可能对治疗糖尿病和肥胖症有用。在8只犬身上植入十二指肠和回肠插管，以确定长期摄入小麦淀粉酶抑制剂是否能维持淀粉酶抑制作用，或是否会影响胃肠功能、代谢功能或胰腺生长。
5只犬进食时喂食1.5克抑制剂，持续9周；3只犬不喂食抑制剂。定期进行餐后及八肽胆囊收缩素刺激后的胰腺分泌研究，以及粪便平衡研究。实验结束后，对胰腺进行分析。
两组体重减轻情况相似。在整个9周内淀粉酶抑制作用持续存在；从餐后第1小时到第6小时，抑制率从91%降至37%。淀粉酶抑制作用在第1小时降低了血浆葡萄糖水平（P < 0.05），增加了碳水化合物向回肠的输送量（315 vs. 555毫克/小时；P = 0.002），并增加了八肽胆囊收缩素刺激的淀粉酶分泌。然而，淀粉酶抑制作用并未显著改变胰岛素、肽YY或神经降压素的血浆浓度、餐后胰腺分泌、胃肠转运或胰腺重量，以及蛋白质或DNA含量。
餐时摄入1.5克抑制剂9周可降低餐后淀粉酶水平，足以延迟碳水化合物的消化和吸收，并降低血浆葡萄糖水平，而不会改变胰腺生长。该剂量可能对治疗糖尿病有效，但对治疗肥胖症无效。
