Vig B K, Latour D, Frankovich J
Department of Biology, University of Nevada, Reno 89557-0015.
J Cell Sci. 1994 Nov;107 ( Pt 11):3091-5. doi: 10.1242/jcs.107.11.3091.
The minor satellite DNA of mouse is believed to constitute the centromere. We report that centromeres of some chromosomes in the Cl1D cells of mouse are not associated with this DNA even though the latter is present on these chromosomes. The satellite DNA was detected distally from the centromere and could not be mistaken as a component of the centromere. We also report that the site of the primary constriction may not always coincide with the site of the anti-kinetochore antibody reaction. Whereas the regions containing the major satellite decondense upon treatment with bisbenzimidazole (Hoechst 33258), the sites carrying minor satellite resist decondensing.
小鼠的小卫星DNA被认为构成了着丝粒。我们报告称，小鼠Cl1D细胞中某些染色体的着丝粒与这种DNA并无关联，即便后者存在于这些染色体上。卫星DNA在着丝粒远端被检测到，不可能被误认为是着丝粒的组成部分。我们还报告称，主缢痕的位置可能并不总是与抗动粒抗体反应的位置重合。在用双苯并咪唑（Hoechst 33258）处理后，含有主要卫星的区域会解聚，而携带小卫星的位点则抵抗解聚。
