McQueen F M, Skinner M A, Krissansen G W, Robinson E, Tan P L
Department of Molecular Medicine, School of Medicine, University of Auckland, New Zealand.
J Rheumatol. 1994 Dec;21(12):2266-73.
To examine the cytotoxic activity of natural killer (NK) cells from peripheral blood (PB) and synovial fluid (SF) of patients with psoriatic arthritis (PsA). The influence of selected inflammatory mediators on the cytolytic function and integrin expression of NK cells was also studied.
Paired samples of PB and SF lymphocytes (PBL and SFL) were obtained from 8 patients with PsA for comparison of NK activity between PBL and SFL. In 6 patients the phenotype of NK cells was determined by flow cytometry using monoclonal antibodies (Mab) to natural killer associated antigen (NKH-1) and the beta 7 integrin, HML-1 (human mucosal lymphocyte adhesion molecule).
NK activity of PB samples was significantly greater than paired SF (p = 0.015). SF NK activity was enhanced by overnight culture with interleukin 2 (IL-2) (p < 0.05). A trend towards reduction of NK activity by prostaglandin E2 (PGE2) was noted (p = 0.06) whereas interleukin 6 (IL-6) and indomethacin had no significant effect. NK activity did not correlate with the percentage of NK cells in PB or SF. However, all SF samples contained a greater proportion of monocytes than PB samples. The expression of HML-1 on NK cells correlated with expression HML-1 on CD3+ cells (r = 0.82) and was greater in SF than PB in PsA and RA patients. Effects of IL-2 on HML-1 expression by NK cells were variable in the 3 patients studied.
In PsA, HML-1 is an activation marker on NK cells. IL-2 expands or maintains the population of HML-1/NKH1 positive cells and increases NK cytolytic activity. However, cytolytic activity of activated NK cells may be inhibited by monocyte derived PGE2.
检测银屑病关节炎（PsA）患者外周血（PB）和滑液（SF）中自然杀伤（NK）细胞的细胞毒性活性。还研究了所选炎症介质对NK细胞溶细胞功能和整合素表达的影响。
从8例PsA患者中获取配对的PB和SF淋巴细胞样本（PBL和SFL），以比较PBL和SFL之间的NK活性。在6例患者中，使用针对自然杀伤相关抗原（NKH-1）和β7整合素HML-1（人黏膜淋巴细胞黏附分子）的单克隆抗体（Mab），通过流式细胞术测定NK细胞的表型。
PB样本的NK活性显著高于配对的SF（p = 0.015）。用白细胞介素2（IL-2）过夜培养可增强SF的NK活性（p < 0.05）。观察到前列腺素E2（PGE2）有降低NK活性的趋势（p = 0.06），而白细胞介素6（IL-6）和吲哚美辛无显著影响。NK活性与PB或SF中NK细胞的百分比无关。然而，所有SF样本中的单核细胞比例均高于PB样本。NK细胞上HML-1的表达与CD3 +细胞上HML-1的表达相关（r = 0.82），在PsA和类风湿关节炎（RA）患者中，SF中的表达高于PB。在所研究的3例患者中，IL-2对NK细胞HML-1表达的影响各不相同。
在PsA中，HML-1是NK细胞上的一种活化标志物。IL-2可扩增或维持HML-1 / NKH1阳性细胞群体并增加NK溶细胞活性。然而，活化NK细胞的溶细胞活性可能受到单核细胞衍生的PGE2的抑制。
