Jorgensen C, Travaglio-Encinoza A, Bologna C, D'Angeac A D, Reme T, Sany J
Service d'Immuno-Rhumatologie, Centre Gui-de-Chauliac, Montpellier, France.
J Rheumatol. 1994 Sep;21(9):1602-7.
Human mucosyl lymphocyte marker (HML-1) antigen is an activation antigen and adhesion molecule of the beta 7 integrin family, which is generally restricted to T cells found in the intestinal epithelium. Expression of the membrane antigen as defined by the monoclonal antibody HML-1 was studied on peripheral blood (PB) lymphocytes and synovial fluid (SF) lymphocytes in 10 patients with rheumatoid arthritis (RA) and in a control group of patients with osteoarthritis (OA).
Double fluorescence activated flow cytometry was used to assess HML-1 expression with T cell subtype antigens (CD3, CD4, CD8) or activation markers interleukin 2 receptor (IL-2R) (CD25), HLA-DR, and lymphocyte function associated antigen (LFA-1) (CD11a) were assessed by flow cytometry.
HML-1 antigen expression in PB lymphocytes of patients with RA (7.3%) was found to be comparable to the control group (6.4%). In contrast, 25.4% (range 14-43%) of SF lymphocytes expressed HML-1 antigen, compared to 13.6% of SF lymphocytes in patients with OA (p < 0.001). In RA, 62% of HML-1 positive cells from SF lymphocytes were the CD8 subtype, compared to 10.6% of PB lymphocytes (p < 0.003), and 18% of control SF lymphocytes (p < 0.05). Furthermore, HML-1 antigen and HLA-DR antigen were coexpressed in 75% of RA SF lymphocytes compared to 29.6% of control SF lymphocytes (p < 0.01). In contrast, coexpression of LFA-1 and the Il-2R did not differ from that of control.
We describe overexpression of the adhesion molecule HML-1 in SF lymphocytes of patients with RA, preferentially in the CD8 subset. These results suggest a similarity between the expression of activation antigens in SF lymphocytes of patients with RA and T lymphocytes present in the intestinal epithelium.
人类黏膜淋巴细胞标志物（HML-1）抗原是β7整合素家族的一种活化抗原和黏附分子，通常局限于肠道上皮中的T细胞。采用单克隆抗体HML-1对10例类风湿关节炎（RA）患者外周血（PB）淋巴细胞和滑液（SF）淋巴细胞以及骨关节炎（OA）患者对照组的膜抗原表达进行了研究。
采用双荧光激活流式细胞术评估HML-1表达，同时通过流式细胞术评估T细胞亚群抗原（CD3、CD4、CD8）或活化标志物白细胞介素2受体（IL-2R）（CD25）、HLA-DR以及淋巴细胞功能相关抗原（LFA-1）（CD11a）。
发现RA患者PB淋巴细胞中HML-1抗原表达（7.3%）与对照组（6.4%）相当。相比之下，25.4%（范围14 - 43%）的SF淋巴细胞表达HML-1抗原，而OA患者SF淋巴细胞中这一比例为13.6%（p < 0.001）。在RA中，SF淋巴细胞中62%的HML-1阳性细胞为CD8亚群，相比之下PB淋巴细胞中这一比例为10.6%（p < 0.003），对照组SF淋巴细胞中为18%（p < 0.05）。此外，75%的RA SF淋巴细胞中HML-1抗原和HLA-DR抗原共表达，而对照组SF淋巴细胞中这一比例为29.6%（p < 0.01）。相比之下，LFA-1和IL-2R的共表达与对照组无差异。
我们描述了RA患者SF淋巴细胞中黏附分子HML-1的过表达，优先出现在CD8亚群中。这些结果表明RA患者SF淋巴细胞中活化抗原的表达与肠道上皮中存在的T淋巴细胞之间存在相似性。
