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Isolation of nucleic acid from paraffin embedded tissue for PCR amplification and sequencing of TcR V beta genes.

作者信息

Hodges E, Smith J

机构信息

Wessex Immunology Service, Southampton University Hospital NHS Trust, U.K.

出版信息

Leuk Res. 1995 Mar;19(3):183-6. doi: 10.1016/0145-2126(94)00148-4.

DOI:10.1016/0145-2126(94)00148-4
PMID:7535371
Abstract

PCR analysis of DNA and RNA from fresh and frozen tissue is now a routine practice in most laboratories. Furthermore DNA extracted from paraffin embedded tissue is routinely used for clonality studies and for HLA typing. However the use of RNA from paraffin embedded tissue is more problematical due to degradation and only short sequences are suitable for amplification. In this report we examine the suitability of extracted nucleic acid from paraffin embedded tissue for the PCR amplification of TcR V beta genes and sequencing of resultant PCR products.

摘要

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