Thomas T E, Lansdorp P M
Terry Fox Laboratory, B.C. Cancer Agency, University of British Columbia, Vancouver, Canada.
Prog Clin Biol Res. 1994;389:65-77.
We have developed a reliable, purely immunological means of quantitatively labeling cells with magnetic colloidal dextran iron. Labeled cells can be efficiently separated with a high gradient magnetic filter inside a magnetic field. Separation conditions and filter design can be changed to accommodate large and small scale positive or negative selection. This separation technique has been applied to tumor cell depletions, stem cell enrichment, and large scale preclinical T-cell depletions.
我们已经开发出一种可靠的、纯免疫学方法，用于用磁性胶体葡聚糖铁对细胞进行定量标记。标记的细胞可以在磁场中用高梯度磁滤器有效地分离。可以改变分离条件和滤器设计，以适应大规模或小规模的阳性或阴性选择。这种分离技术已应用于肿瘤细胞清除、干细胞富集和大规模临床前T细胞清除。
