Rubinstein I, Müns G, Zucker I H
Department of Medicine, University of Illinois at Chicago.
Basic Res Cardiol. 1994 Sep-Oct;89(5):487-98. doi: 10.1007/BF00788284.
The purpose of this study was to determine the effects of bradykinin (BK), substance P (SP) and histamine on plasma exudation in the skin of conscious dogs with and without pacing-induced heart failure. We also determined the role tissue angiotensin I-converting enzyme (ACE) and neutral endopeptidase (NEP) play in modulating these responses. We found that intradermal injection of BK, SP and histamine induced a significant, concentration-dependent Evans blue exudation in normal dogs (p < 0.05). Bradykinin-induced responses were significantly potentiated by captopril (p < 0.05). In contrast, phosphoramidon potentiated BK-induced responses only at low concentrations of BK. Both captopril and phosphoramidon had no significant effects on SP- and histamine-induced Evans blue exudation. BK- and SP-induced responses were significantly attenuated, whereas histamine-induced Evans blue exudation was significantly potentiated in dogs with heart failure. We conclude that heart failure is associated with attenuation of BK- and SP-, but not histamine-induced plasma exudation in the peripheral microcirculation and that these responses are not modulated by tissue ACE and NEP.
本研究的目的是确定缓激肽（BK）、P物质（SP）和组胺对伴有和不伴有起搏诱导性心力衰竭的清醒犬皮肤血浆渗出的影响。我们还确定了组织血管紧张素I转换酶（ACE）和中性内肽酶（NEP）在调节这些反应中所起的作用。我们发现，在正常犬中，皮内注射BK、SP和组胺可诱导显著的、浓度依赖性的伊文思蓝渗出（p < 0.05）。卡托普利可显著增强缓激肽诱导的反应（p < 0.05）。相比之下，磷酰胺素仅在低浓度缓激肽时增强其诱导的反应。卡托普利和磷酰胺素对SP和组胺诱导的伊文思蓝渗出均无显著影响。在心力衰竭犬中，BK和SP诱导的反应显著减弱，而组胺诱导的伊文思蓝渗出显著增强。我们得出结论，心力衰竭与外周微循环中BK和SP诱导的血浆渗出减弱有关，但与组胺无关，并且这些反应不受组织ACE和NEP的调节。
