环磷酸腺苷（cAMP）和钙离子（Ca²⁺）依赖的细胞内机制在体外对牛卵巢颗粒细胞催产素和血管加压素分泌的控制中的作用。
The role of cAMP- and Ca(2+)-dependent intracellular mechanisms in the control of oxytocin and vasopressin secretion by bovine ovarian granulosa cells in vitro.
作者信息
Sirotkin A V, Nitray J
机构信息
Research Institute of Animal Production, Nitra, Slovakia.
出版信息
Biol Signals. 1994 Sep-Oct;3(5):239-46. doi: 10.1159/000109551.
The secretion of oxytocin and arginine-vasopressin was demonstrated in bovine granulosa cell culture. It was found that dbcAMP or 3-isobutyl-1-methyl-xanthine (an inhibitor of intracellular cAMP metabolization) additions increased both oxytocin and vasopressin release. The Ca2+ ionophore A23187 also stimulated, while the Ca2+ channel blocker verapamil inhibited the secretion of both nonapeptide hormones. These results suggest the involvement of cAMP- and Ca(2+)-dependent intracellular mechanisms in the stimulation of both oxytocin and vasopressin secretion by bovine granulosa cells.
在牛颗粒细胞培养中证实了催产素和精氨酸加压素的分泌。发现添加二丁酰环磷腺苷（dbcAMP）或3-异丁基-1-甲基黄嘌呤（一种细胞内cAMP代谢抑制剂）会增加催产素和加压素的释放。钙离子载体A23187也能刺激这两种九肽激素的分泌，而钙离子通道阻滞剂维拉帕米则抑制其分泌。这些结果表明，cAMP和Ca(2+)依赖性细胞内机制参与了牛颗粒细胞对催产素和加压素分泌的刺激。
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