Sengeløv H, Follin P, Kjeldsen L, Lollike K, Dahlgren C, Borregaard N
Department of Hematology, National University Hospital, Rigshospitalet, Copenhagen, Denmark.
J Immunol. 1995 Apr 15;154(8):4157-65.
The extent of mobilization of four different intracellular compartments was measured during in vivo exudation of neutrophils into skin chambers and compared with resting neutrophils obtained from blood. Exudation of neutrophils induced increased surface expression of alkaline phosphatase, complement receptor 1, and Mac-1, and a complete loss of L-selectin. The increase in the content of surface molecules in the plasma membrane is in accordance with complete mobilization of secretory vesicles. Granule matrix proteins were secreted into the chamber fluid by the exudated neutrophils and the exocytosed proteins were recovered in the skin chamber fluid. Release of gelatinase from gelatinase granules was 38.1%, lactoferrin release from specific granules was 21.9%, and myeloperoxidase release from azurophil granules was 7.0%, clearly illustrating a hierarchy in mobilization among granules. When exudate neutrophils were stimulated with FMLP, additional mobilization of granules was observed and the rank order regarding release was preserved. This is the first report to evaluate the mobilization of secretory vesicles during in vivo exudation of human neutrophils. It is shown that secretory vesicles are regulated exocytotic vesicles that are fully mobilized during in vivo exudation. Once exocytosed, secretory vesicles are not re-formed within a period of 6 h.
在中性粒细胞体内渗出到皮肤小室的过程中，测量了四种不同细胞内区室的动员程度，并与从血液中获取的静息中性粒细胞进行了比较。中性粒细胞的渗出导致碱性磷酸酶、补体受体1和Mac-1的表面表达增加，以及L-选择素完全丧失。质膜中表面分子含量的增加与分泌囊泡的完全动员一致。颗粒基质蛋白由渗出的中性粒细胞分泌到小室液中，胞吐的蛋白在皮肤小室液中被回收。明胶酶颗粒中明胶酶的释放率为38.1%，特异性颗粒中乳铁蛋白的释放率为21.9%，嗜天青颗粒中髓过氧化物酶的释放率为7.0%，清楚地表明了颗粒间动员的层级关系。当用FMLP刺激渗出的中性粒细胞时，观察到颗粒的额外动员，且释放的排序得以保留。这是第一份评估人类中性粒细胞体内渗出过程中分泌囊泡动员情况的报告。结果表明，分泌囊泡是受调节的胞吐囊泡，在体内渗出过程中被完全动员。一旦胞吐，分泌囊泡在6小时内不会重新形成。
