McKenzie K E, Bandyopadhyay G K, Imagawa W, Sun K, Nandi S
Department of Molecular and Cell Biology, University of California at Berkeley 94720.
Prostaglandins Leukot Essent Fatty Acids. 1994 Dec;51(6):437-43. doi: 10.1016/0952-3278(94)90062-0.
The direct effect of omega-3 and omega-6 fatty acids on the proliferation of mouse mammary tumor cells (MTC) was examined in a serum-free cell culture system. While the EGF-induced proliferation of normal mammary epithelial cells was shown to be enhanced by omega-3 and omega-6 fatty acids and prostaglandins (PGs), a majority (75-80%) of primary mammary tumors were not stimulated by these agents. Compared to normal cells, some MTC cultures showed a higher susceptibility to inhibition by omega-3 fatty acids. The general lack of response of MTC cultures to PGE2 and cyclic adenosine monophosphate (cAMP) suggests some alterations in the cAMP-mediated pathway. However, the PGE2-induced cAMP levels and cAMP-dependent protein kinase (PKA) activities in the tumor cells were comparable to normal cells. We conclude that the proliferation of mammary tumor cells either follow a cAMP-PKA-independent pathway or have some alterations in the serine/threonine kinase mediated signaling pathway.
在无血清细胞培养系统中检测了ω-3和ω-6脂肪酸对小鼠乳腺肿瘤细胞（MTC）增殖的直接影响。虽然ω-3和ω-6脂肪酸以及前列腺素（PGs）可增强表皮生长因子诱导的正常乳腺上皮细胞增殖，但大多数（75%-80%）原发性乳腺肿瘤不受这些因子刺激。与正常细胞相比，一些MTC培养物对ω-3脂肪酸抑制作用的敏感性更高。MTC培养物对前列腺素E2（PGE2）和环磷酸腺苷（cAMP）普遍缺乏反应，提示cAMP介导途径存在某些改变。然而，肿瘤细胞中PGE2诱导的cAMP水平和cAMP依赖性蛋白激酶（PKA）活性与正常细胞相当。我们得出结论，乳腺肿瘤细胞的增殖要么遵循不依赖cAMP-PKA的途径，要么在丝氨酸/苏氨酸激酶介导的信号通路中存在某些改变。
