Limat A, Breitkreutz D, Thiekoetter G, Klein C E, Braathen L R, Hunziker T, Fusenig N E
Department of Dermatology, Inselspital, University of Berne, Switzerland.
Transplantation. 1995 Apr 15;59(7):1032-8. doi: 10.1097/00007890-199504150-00020.
The outer root sheath of hair follicles mainly consists of basal-like keratinocytes which can substitute for interfollicular epidermal keratinocytes, as during healing of skin wounds when outer root sheath cells migrate onto the denuded area, thus contributing to epidermal regeneration. Human outer root sheath cells represent a repeatedly available source of keratinocytes which can be easily and extensively expanded in culture. Close comparison of organotypic cultures of either outer root sheath cells or epidermal keratinocytes grafted onto nude mice demonstrated that outer root sheath cells formed a stratified epithelium resembling normal epidermis that is virtually indistinguishable from that developed by epidermal keratinocytes. Typical epidermal differentiation markers, such as the suprabasal keratins 1 and 10, involucrin, filaggrin, the basement membrane components collagen type IV and laminin, and the integrin chains alpha 2, alpha 3, alpha 6, and beta 1, were readily expressed in a mostly regular localization. These data suggest that outer root sheath cells, bearing essential advantages as compared with interfollicular keratinocytes, are suitable for skin replacement.
毛囊外根鞘主要由基底样角质形成细胞组成，这些细胞可替代毛囊间表皮角质形成细胞，例如在皮肤伤口愈合过程中，外根鞘细胞迁移到裸露区域，从而促进表皮再生。人外根鞘细胞是角质形成细胞的一个可反复获取的来源，能够在培养中轻松且大量地扩增。将外根鞘细胞或表皮角质形成细胞移植到裸鼠上进行的器官型培养的密切比较表明，外根鞘细胞形成了类似于正常表皮的分层上皮，与表皮角质形成细胞形成的上皮几乎无法区分。典型的表皮分化标志物，如基底层以上的角蛋白1和10、内披蛋白、丝聚蛋白、基底膜成分IV型胶原蛋白和层粘连蛋白，以及整合素链α2、α3、α6和β1，大多以规则的定位易于表达。这些数据表明，与毛囊间角质形成细胞相比具有重要优势的外根鞘细胞适合用于皮肤替代。
