Hao F, Li M D
Department of Infectious Diseases, First Hospital, Third Military Medical University, Chongqing.
Zhonghua Nei Ke Za Zhi. 1994 Sep;33(9):593-6.
Techniques of enzyme-linked immunosorbent assay based on synthetic multiple peptide fragments and second generation recombinant immunoblot assay (RIBA) were used to study the patterns of specific antibody response in 10 cases of posttransfusion hepatitis (PTH) during a period of 36-38 weeks after blood transfusion. Nine cases were positive with serum anti-HCV, including 8 cases positive with serum HCV-RNA. Antibodies to core protein of HCV showed a higher positive rate and were detected 1-3 weeks earlier than those to the putative nonstructural (NS) protein. Anti-HCV IgM to core protein were detected 1-4 weeks earlier than anti-HCV IgG and the detective absorbent values of anti-HCV IgM were positively correlated with the levels of serum ALT (P < 0.01). "Passive transfer" of anti-HCV were found in 3 cases. These facts suggest that HCV is the major causative agent of PTH cases in our district and anti-HCV IgM to core protein is a putative serological marker not only for early diagnosis of HCV infection but also for demonstration of active HCV infection.
采用基于合成多肽片段的酶联免疫吸附测定技术和第二代重组免疫印迹法（RIBA），对10例输血后肝炎（PTH）患者输血后36 - 38周期间的特异性抗体反应模式进行了研究。9例血清抗-HCV阳性，其中8例血清HCV-RNA阳性。抗HCV核心蛋白抗体阳性率较高，比推定的非结构（NS）蛋白抗体早1 - 3周检测到。抗HCV核心蛋白IgM比抗HCV IgG早1 - 4周检测到，抗HCV IgM的检测吸光度值与血清ALT水平呈正相关（P < 0.01）。3例发现抗-HCV的“被动转移”。这些事实表明，HCV是我区PTH病例的主要致病因子，抗HCV核心蛋白IgM不仅是HCV感染早期诊断的推定血清学标志物，也是活动性HCV感染的标志物。
