[Effect of cultured mice hepatocytes on the proliferation of immunocompetent cells].

Mice hepatocytes in primary cultures were treated during 2 h with normal rabbit antibodies (0.5 mg/ml), antiliver antibodies (0.5 mg/ml) and CCl4 (5 mM). Functional state of cells was studied according to DNA, RNA and protein synthesis. Cell-free supernatants were collected during 24 h after cells treatment. Spontaneous proliferation in vitro of syngeneic cells from the spleen, thymus, lymph node and bone marrow cultured with hepatocyte supernatants was investigated. Cultured intact hepatocytes were shown to produce factors that changed proliferation of the thymus and spleen cells. Hepatocyte supernatants demonstrated different effect on cells from different immune organs. Hepatocyte function alteration (minimal under normal antibodies treatment and maximal under CCl4 treatment) changed liver cells humoral influence on the immune cells.