Makohon N V, Aleksieieva I M
Fiziol Zh (1994). 1994 Jan-Feb;40(1):77-82.
Mice hepatocytes in primary cultures were treated during 2 h with normal rabbit antibodies (0.5 mg/ml), antiliver antibodies (0.5 mg/ml) and CCl4 (5 mM). Functional state of cells was studied according to DNA, RNA and protein synthesis. Cell-free supernatants were collected during 24 h after cells treatment. Spontaneous proliferation in vitro of syngeneic cells from the spleen, thymus, lymph node and bone marrow cultured with hepatocyte supernatants was investigated. Cultured intact hepatocytes were shown to produce factors that changed proliferation of the thymus and spleen cells. Hepatocyte supernatants demonstrated different effect on cells from different immune organs. Hepatocyte function alteration (minimal under normal antibodies treatment and maximal under CCl4 treatment) changed liver cells humoral influence on the immune cells.
原代培养的小鼠肝细胞分别用正常兔抗体（0.5毫克/毫升）、抗肝抗体（0.5毫克/毫升）和四氯化碳（5毫摩尔）处理2小时。根据DNA、RNA和蛋白质合成情况研究细胞的功能状态。细胞处理后24小时收集无细胞上清液。研究了用肝细胞上清液培养的来自脾脏、胸腺、淋巴结和骨髓的同基因细胞在体外的自发增殖情况。结果显示，培养的完整肝细胞可产生改变胸腺和脾脏细胞增殖的因子。肝细胞上清液对来自不同免疫器官的细胞表现出不同的作用。肝细胞功能改变（正常抗体处理时最小，四氯化碳处理时最大）改变了肝细胞对免疫细胞的体液影响。
