Embryonic development of the antennal lobes of a hemimetabolous insect, the cockroach Periplaneta americana: light and electron microscopic observations.

In the hemimetabolous insect Periplaneta americana, the adult-like organization of the primary olfactory centers, the antennal lobes, is established during the approximately 31 days of embryogenesis. This report describes the temporal sequence of developmental events as viewed in the light and electron microscope by means of histological stains and by DiI labeling of antennal receptor axons with subsequent photoconversion. Glomeruli, characteristic differentiations of the antennal lobe neuropil, are first observed on day 19; their development, which is not synchronous in the various parts of the antennal lobe, lasts until about day 22. From day 10 on, glial cells begin to form a narrow boundary layer between the soma cortex and the central neuropil. They exhibit a lengthening of their processes in parallel with the formation of glomeruli. Marked proliferation or migration of these glial cells into the neuropil between glomeruli has not been observed. Antennal receptor axons could be labeled from stage 15 on. They terminate in an elongated growth cone with numerous filopodia. From day 18 on, some of these become bent or show an initial bifurcation. From day 22 on, the first afferent axons develop an adult-like arborization pattern. Synaptic contacts between receptor axons and unidentified neurons were observed as early as stages 16 and 19, in which the axons still have a growth cone-like form. In stage 27, in which the fibers have adult-like arborizations, many output contacts and few input contacts were found.