Kasper M, Müller M
Institute of Pathology, Technical University Dresden, Germany.
Acta Histochem. 1994 Dec;96(4):439-44. doi: 10.1016/S0065-1281(11)80030-9.
The expression of pan-cadherin was investigated immunohistochemically in normal and irradiated mini pig lungs. Using a double labelling immunofluorescence technique, pan-cadherin expression was also compared with that of cytokeratin 18, which is selectively present in type II cells. In untreated animals pan-cadherin was detected in type I pneumocytes, in alveolar macrophages and in endothelial cells of larger blood vessels. Radiation caused increased pan-cadherin immunoreactivity of bronchial epithelial cells and interstitial cells. In addition, pan-cadherin expression was induced in type II pneumocytes. These changes in the distribution of epithelial cadherin molecules may implicate a role of cadherins for epithelial remodeling in response to radiation-induced lung injury.
采用免疫组织化学方法研究了正常和辐照小型猪肺组织中泛钙黏蛋白的表达情况。运用双标记免疫荧光技术，还将泛钙黏蛋白的表达与细胞角蛋白18（其选择性地存在于II型细胞中）的表达进行了比较。在未接受治疗的动物中，I型肺细胞、肺泡巨噬细胞以及较大血管的内皮细胞中均可检测到泛钙黏蛋白。辐射导致支气管上皮细胞和间质细胞的泛钙黏蛋白免疫反应性增强。此外，II型肺细胞中也诱导出了泛钙黏蛋白的表达。上皮钙黏蛋白分子分布的这些变化可能暗示钙黏蛋白在辐射诱导的肺损伤后上皮重塑过程中发挥作用。
