Arvidsson J, Fundin B T, Pfaller K
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
J Comp Neurol. 1995 Jan 23;351(4):489-98. doi: 10.1002/cne.903510402.
The innervation of the rat hard palate and the bordering part of the soft palate was studied after anterograde transport of horseradish peroxidase conjugated to wheat germ agglutinin (WGA-HRP) and to choleragenoid (B-HRP) in separate experiments. WGA-HRP labeling showed leakage from several types of nerve endings, whereas B-HRP did not. Both conjugates gave rise to heavy labeling of a variety of nerve endings. Intragemmal and, especially, perigemmal fibers were labeled in chemosensory corpuscles, which were most common in the medial wall of the incisive canal and in the most anterior part of the soft palate. Ruffini endings of different sizes were labeled in the incisive papilla. Other subepithelial endings forming elongated expanded profiles with medium- to large-caliber source fibers were most common in protruding parts of the palate. Labeled intraepithelial endings included Merkel endings, which were most frequent in the incisive papilla and the rugae. Other labeled profiles were medium-caliber afferents giving rise to irregular, beaded, and sometimes branched endings often located far superficially in the epithelium. Such endings were present both within and between protruding parts of the palate. Fine-caliber intraepithelial endings were labeled almost exclusively in WGA-HRP experiments.
在分别进行的实验中，通过将与小麦胚凝集素（WGA-HRP）和霍乱毒素（B-HRP）偶联的辣根过氧化物酶进行顺行运输后，研究了大鼠硬腭和软腭相邻部分的神经支配情况。WGA-HRP标记显示几种类型的神经末梢有渗漏现象，而B-HRP则没有。两种偶联物都使多种神经末梢产生了大量标记。化学感觉小体中的内芽纤维，尤其是芽周纤维被标记，这些小体在切牙管的内侧壁和软腭的最前部最为常见。不同大小的鲁菲尼终末在切牙乳头中被标记。其他形成细长扩张轮廓且具有中到大口径源纤维的上皮下终末在腭的突出部分最为常见。标记的上皮内终末包括默克尔终末，其在切牙乳头和皱襞中最为常见。其他标记的轮廓是中口径传入纤维，产生不规则、串珠状且有时分支的终末，这些终末通常位于上皮的浅表位置。这种终末在腭的突出部分内部和之间都有。细口径上皮内终末几乎仅在WGA-HRP实验中被标记。
