de Fabritis P, Amadori S, Petti M C, Mancini M, Montefusco E, Picardi A, Geiser T, Campbell K, Calabretta B, Mandelli F
Department of Human Biopathology, University 'La Sapienza' Rome, Italy.
Leukemia. 1995 Apr;9(4):662-4.
We treated a patient with chronic myeloid leukaemia in accelerated phase with autologous bone marrow transplantation. Before reinfusion, cells were purged in vitro with a 26-mer phosphorothioate antisense oligodeoxynucleotide specific for the B2A2 junction. Incubation with antisense oligodeoxynucleotides produced a 24 and 41% reduction of CFU-GM and CD34+ cells, respectively. However, an in vitro test previously performed as a screening for patient inclusion in this procedure, revealed a 38 and 75% reduction of colony formation after 24-h and 168-h incubation, respectively. The patient showed bone marrow engraftment 15 days after reinfusion and haematological reconstitution after 17 and 25 days for platelets and neutrophils, respectively. Using fluorescence in situ hybridization in interphase nuclei, we demonstrated the presence of a proportion of Ph-negative cells in repeated controls after the autograft. The patient is now in unmaintained complete haematological remission 9 months after the autograft.
我们对一名处于加速期的慢性髓性白血病患者进行了自体骨髓移植治疗。在回输前，使用针对B2A2连接区的26聚硫代磷酸酯反义寡脱氧核苷酸在体外对细胞进行净化处理。与反义寡脱氧核苷酸孵育分别使CFU - GM和CD34 +细胞减少了24%和41%。然而，先前作为该程序患者纳入筛选进行的体外试验显示，孵育24小时和168小时后集落形成分别减少了38%和75%。患者在回输后15天出现骨髓植入，血小板和中性粒细胞分别在17天和25天后实现血液学重建。通过间期核荧光原位杂交，我们在自体移植后的重复对照中证实存在一定比例的Ph阴性细胞。该患者在自体移植9个月后处于未维持的完全血液学缓解状态。
