Bergson C, Mrzljak L, Lidow M S, Goldman-Rakic P S, Levenson R
Department of Pharmacology, Pennsylvania State College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3468-72. doi: 10.1073/pnas.92.8.3468.
To achieve a better understanding of how D5 dopamine receptors mediate the actions of dopamine in brain, we have developed antibodies specific for the D5 receptor. D5 antibodies reacted with recombinant baculovirus-infected Sf9 cells expressing the D5 receptor but not with the D1 receptor or a variety of other catecholaminergic and muscarinic receptors. Epitope-tagged D5 receptors expressed in mammalian cells were reactive with both D5 antibodies and an epitope-specific probe. A mixture of N-linked glycosylated polypeptides and higher molecular-mass species was detected on immunoblots of membrane fractions of D5-transfected cells and also of primate brain. D5 receptor antibodies intensely labeled pyramidal neurons in the prefrontal cortex, whereas spiny medium-sized neurons and aspiny large interneurons of the caudate nucleus were relatively lightly labeled. Antibodies to the D5 dopamine receptor should prove important in experimentally determining specific roles for the D5 and D1 receptors in cortical processes and diseases.
为了更好地理解D5多巴胺受体如何介导多巴胺在大脑中的作用，我们开发了针对D5受体的特异性抗体。D5抗体与表达D5受体的重组杆状病毒感染的Sf9细胞发生反应，但不与D1受体或多种其他儿茶酚胺能和毒蕈碱受体发生反应。在哺乳动物细胞中表达的表位标记的D5受体与D5抗体和表位特异性探针均有反应。在D5转染细胞以及灵长类动物大脑的膜部分免疫印迹中检测到N-连接糖基化多肽和更高分子量物质的混合物。D5受体抗体强烈标记前额叶皮质中的锥体神经元，而尾状核的棘状中型神经元和无棘大型中间神经元标记相对较轻。D5多巴胺受体抗体在实验确定D5和D1受体在皮质过程和疾病中的特定作用方面应具有重要意义。
