血小板活化因子拮抗剂对雨蛙肽诱导的胰腺炎的影响。

Effect of PAF antagonists on cerulein-induced pancreatitis.

作者信息

Jancar S, Abdo E E, Sampietre S N, Kwasniewski F H, Coelho A M, Bonizzia A, Machado M C

机构信息

Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Brazil.

出版信息

J Lipid Mediat Cell Signal. 1995 Jan;11(1):41-9. doi: 10.1016/0929-7855(94)00026-9.

DOI:10.1016/0929-7855(94)00026-9

PMID:7537159

Abstract

The present study was undertaken to investigate the involvement of PAF in acute pancreatitis induced by cerulein in rats. Cerulein (two doses of 20 micrograms/rat, the first s.c. and the second i.v., 1 h apart) induced a significant increase in vascular permeability in the pancreas, evaluated by the Evans blue (EB) extravasation method. Plasma amylase levels were also significantly increased in this group. The PAF antagonists, BN-52021 (5 mg/kg) and WEB-2170 (1 and 10 mg/kg), both significantly reduced the extravasation of EB in the pancrease induced by i.v. injection of PAF (1 microgram/kg). At these concentrations, BN-52021 was effective at inhibiting cerulein-induced pancreatitis (60-70% of inhibition) whereas WEB-2170 had no significant effect. Although the inhibition induced by BN-52021 suggests the involvement of PAF in cerulein-pancreatitis, the lack of effect of WEB-2170 reported here does not allow a definite conclusion. Further studies are needed to elucidate the differential effect of the PAF antagonists.

摘要

本研究旨在探讨血小板活化因子（PAF）在雨蛙肽诱导的大鼠急性胰腺炎中的作用。雨蛙肽（20微克/大鼠，分两次给药，第一次皮下注射，第二次静脉注射，间隔1小时）通过伊文思蓝（EB）外渗法评估，可使胰腺血管通透性显著增加。该组血浆淀粉酶水平也显著升高。PAF拮抗剂BN - 52021（5毫克/千克）和WEB - 2170（1毫克/千克和10毫克/千克）均能显著降低静脉注射PAF（1微克/千克）诱导的胰腺EB外渗。在这些浓度下，BN - 52021能有效抑制雨蛙肽诱导的胰腺炎（抑制率为60 - 70%），而WEB - 2170则无显著作用。尽管BN - 52021诱导的抑制作用表明PAF参与了雨蛙肽诱导的胰腺炎，但此处报道的WEB - 2170无作用的情况无法得出明确结论。需要进一步研究以阐明PAF拮抗剂的不同作用效果。