Korchev Y E, Alder G M, Bakhramov A, Bashford C L, Joomun B S, Sviderskaya E V, Usherwood P N, Pasternak C A
Department of Cellular & Molecular Sciences, St George's Hospital Medical School, London, United Kingdom.
J Membr Biol. 1995 Jan;143(2):143-51. doi: 10.1007/BF00234660.
The conductance of pores induced by Staphylococcus aureus alpha-toxin in Lettre cells has been compared to that in bilayers composed of synthetic lipids or Lettre cell membrane constituents. Previously described characteristics of toxin-induced conductance changes in lipid bilayers, namely rectification, voltage-dependent closure, and closure at low pH or in the presence of divalent cations (Menestrina, 1986) are displayed also in bilayers prepared from Lettre cell membranes and in patch clamped Lettre cells. It is concluded that endogenous proteins do not affect the properties of alpha-toxin-induced channels significantly and that the relative lack of ion channels in Lettre cells makes them ideal for studies of pore-forming toxins by the patch clamp technique.
已将金黄色葡萄球菌α毒素在Lettre细胞中诱导形成的孔道电导，与在由合成脂质或Lettre细胞膜成分构成的双层膜中的电导进行了比较。先前描述的脂质双层膜中毒素诱导的电导变化特征，即整流、电压依赖性关闭以及在低pH值或存在二价阳离子时关闭（Menestrina，1986），在由Lettre细胞膜制备的双层膜以及膜片钳记录的Lettre细胞中也有表现。得出的结论是，内源性蛋白质不会显著影响α毒素诱导的通道特性，并且Lettre细胞中相对缺乏离子通道，这使得它们成为通过膜片钳技术研究成孔毒素的理想对象。
