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B细胞受体CD22对CD45结合的调节

Regulation of CD45 engagement by the B-cell receptor CD22.

作者信息

Sgroi D, Koretzky G A, Stamenkovic I

机构信息

Department of Pathology, Massachusetts General Hospital, Boston, USA.

出版信息

Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):4026-30. doi: 10.1073/pnas.92.9.4026.

DOI:10.1073/pnas.92.9.4026
PMID:7537381
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC42095/
Abstract

The B-cell receptor CD22 binds sialic acid linked alpha-2-6 to terminal galactose residues on N-linked oligosaccharides associated with several cell-surface glycoproteins. The first of these sialoglycoproteins to be identified was the receptor-linked phosphotyrosine phosphatase CD45, which is required for antigen/CD3-induced T-cell activation. In the present work, we examine the effect of interaction between the extracellular domain of CD45 and CD22 on T-cell activation. Using soluble CD22-immunoglobulin fusion proteins and T cells expressing wild-type and chimeric CD45 forms, we show that engagement of CD45 by soluble CD22 can modulate early T-cell signals in antigen receptor/CD3-mediated stimulation. We also show that addition of sialic acid by beta-galactoside alpha-2,6-sialyltransferase to the CD22 molecule abrogates interactions between CD22 and its ligands. Together, these observations provide direct evidence for a functional role of the interaction between the extracellular domain of CD45 and a natural ligand and suggest another regulatory mechanism for CD22-mediated ligand engagement.

摘要

B细胞受体CD22可与唾液酸结合，唾液酸通过α-2-6连接到与几种细胞表面糖蛋白相关的N-连接寡糖的末端半乳糖残基上。这些唾液酸糖蛋白中第一个被鉴定出来的是受体连接的磷酸酪氨酸磷酸酶CD45，它是抗原/CD3诱导的T细胞活化所必需的。在本研究中，我们研究了CD45的胞外结构域与CD22之间的相互作用对T细胞活化的影响。利用可溶性CD22-免疫球蛋白融合蛋白以及表达野生型和嵌合型CD45形式的T细胞，我们发现可溶性CD22与CD45的结合能够在抗原受体/CD3介导的刺激中调节早期T细胞信号。我们还表明，β-半乳糖苷α-2,6-唾液酸转移酶将唾液酸添加到CD22分子上可消除CD22与其配体之间的相互作用。这些观察结果共同为CD45的胞外结构域与天然配体之间相互作用的功能作用提供了直接证据，并提示了CD22介导的配体结合的另一种调节机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/42095/4fafa0ae81d5/pnas01493-0404-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/42095/00d9bf9fc161/pnas01493-0403-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/42095/4fafa0ae81d5/pnas01493-0404-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/42095/00d9bf9fc161/pnas01493-0403-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/603a/42095/4fafa0ae81d5/pnas01493-0404-a.jpg

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本文引用的文献

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Ligand-mediated negative regulation of a chimeric transmembrane receptor tyrosine phosphatase.配体介导的嵌合跨膜受体酪氨酸磷酸酶的负调控
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Rescue of signaling by a chimeric protein containing the cytoplasmic domain of CD45.通过含有CD45胞质结构域的嵌合蛋白进行信号拯救。
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CD22 associates with the human surface IgM-B-cell antigen receptor complex.CD22与人类表面IgM-B细胞抗原受体复合物相关联。
糖基化的协调变化通过 CD22 调节生发中心。
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N-Linked Glycosylation Regulates CD22 Organization and Function.N-连接糖基化调控 CD22 的结构与功能。
Front Immunol. 2019 Apr 4;10:699. doi: 10.3389/fimmu.2019.00699. eCollection 2019.
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B Cell Siglecs-News on Signaling and Its Interplay With Ligand Binding.B 细胞信号识别分子——信号转导及其与配体结合的最新研究进展。
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An extracatalytic function of CD45 in B cells is mediated by CD22.CD45在B细胞中的一种非催化功能是由CD22介导的。
Proc Natl Acad Sci U S A. 2015 Nov 24;112(47):E6515-24. doi: 10.1073/pnas.1519925112. Epub 2015 Nov 11.
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A novel epitope from CD22 regulates Th1 and Th17 cell function in systemic lupus erythematosus.CD22 上的一个新表位调节系统性红斑狼疮中的 Th1 和 Th17 细胞功能。
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Analysis of Tn antigenicity with a panel of new IgM and IgG1 monoclonal antibodies raised against leukemic cells.分析针对白血病细胞产生的一组新的 IgM 和 IgG1 单克隆抗体的 Tn 抗原性。
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CD45-associated kinase activity requires lck but not T cell receptor expression in the Jurkat T cell line.在Jurkat T细胞系中，CD45相关激酶活性需要lck，但不需要T细胞受体表达。
J Biol Chem. 1993 Apr 25;268(12):8958-64.
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Natural ligands of the B cell adhesion molecule CD22 beta carry N-linked oligosaccharides with alpha-2,6-linked sialic acids that are required for recognition.B细胞黏附分子CD22β的天然配体带有N-连接寡糖，其具有识别所需的α-2,6-连接唾液酸。
J Biol Chem. 1993 Apr 5;268(10):7019-27.
6
CD22, a B cell-specific immunoglobulin superfamily member, is a sialic acid-binding lectin.CD22是一种B细胞特异性免疫球蛋白超家族成员，是一种唾液酸结合凝集素。
J Biol Chem. 1993 Apr 5;268(10):7011-8.
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Inositol trisphosphate and calcium signalling.肌醇三磷酸与钙信号传导
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Structural study of the sugar chains of human leukocyte common antigen CD45.人类白细胞共同抗原CD45糖链的结构研究
Biochemistry. 1993 Nov 30;32(47):12694-704. doi: 10.1021/bi00210a019.
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The B-cell adhesion molecule CD22 is cross-species reactive and recognizes distinct sialoglycoproteins on different functional T-cell sub-populations.B细胞黏附分子CD22具有种间反应性，可识别不同功能性T细胞亚群上不同的唾液酸糖蛋白。
Scand J Immunol. 1994 May;39(5):433-8. doi: 10.1111/j.1365-3083.1994.tb03397.x.
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Sialylation of the B lymphocyte molecule CD22 by alpha 2,6-sialyltransferase is implicated in the regulation of CD22-mediated adhesion.α2,6-唾液酸转移酶对B淋巴细胞分子CD22的唾液酸化作用与CD22介导的黏附调节有关。
J Biol Chem. 1994 Apr 22;269(16):11783-6.