Arpin C, Déchanet J, Van Kooten C, Merville P, Grouard G, Brière F, Banchereau J, Liu Y J
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
Science. 1995 May 5;268(5211):720-2. doi: 10.1126/science.7537388.
After germinal center B cells undergo somatic mutation and antigen selection, they become either memory B cells or plasma cells, but the signal requirements that control entry into either pathway have been unclear. When purified human germinal center cells were cultured with interleukin-2, interleukin-10, and cells expressing CD40 ligand, cells with characteristics of memory B cells were generated. Removal of CD40 ligand from the system resulted in terminal differentiation of germinal center B cells into cells with the characteristics of plasma cells. These results indicate that CD40 ligand directs the differentiation of germinal center B cells toward memory B cells rather than toward plasma cells.
生发中心B细胞经历体细胞突变和抗原选择后，会分化为记忆B细胞或浆细胞，但控制进入这两条分化途径的信号需求尚不清楚。当将纯化的人类生发中心细胞与白细胞介素-2、白细胞介素-10以及表达CD40配体的细胞一起培养时，会产生具有记忆B细胞特征的细胞。从培养体系中去除CD40配体后，生发中心B细胞会终末分化为具有浆细胞特征的细胞。这些结果表明，CD40配体引导生发中心B细胞分化为记忆B细胞而非浆细胞。
